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SRP3321

Sigma-Aldrich

MIF human

recombinant, expressed in insect cells, ≥98% (SDS-PAGE), ≥98% (HPLC)

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Synonym(s):
GIF, GLIF, MMIF
NACRES:
NA.32

biological source

human

recombinant

expressed in insect cells

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

15.0 kDa

packaging

pkg of 25 μg

impurities

endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MIF(4282)

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This Item
SAB5700736WH0004282M1GF180
vibrant-m

SRP3321

MIF human

vibrant-m

GF180

MIF,
Human Recombinant

biological source

human

biological source

rabbit

biological source

mouse

biological source

-

recombinant

expressed in insect cells

recombinant

-

recombinant

-

recombinant

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

UniProt accession no.

P14174

UniProt accession no.

P14174

UniProt accession no.

P14174

UniProt accession no.

P14174

packaging

pkg of 25 μg

packaging

-

packaging

-

packaging

-

General description

Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through cluster of differentiation 74 and 44 (CD74 and CD44), resulting in the secretion of tumor necrosis-α (TNF-α), interleukins-1, -6, -8, and various matrix metalloproteinases (MMPs). The gene encoding this protein is localized on human chromosome 22q11.23. Insect cell derived recombinant MIF is a 15kDa protein containing 124 amino acid residues, including an N-terminal His-tag.

Biochem/physiol Actions

The enzymatic activity of macrophage migration inhibitory factor (MIF) is characterized by its ability to act as a tautomerase, capable of catalyzing the keto to enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue.

Physical form

Lyophilized from 10mM Sodium Phosphate, pH 7.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Dual role of macrophage migration inhibitory factor (MIF) in human breast cancer.
Verjans E
BMC Cancer, 9, 230-230 (2009)
Inhibition of MIF bioactivity by rational design of pharmacological inhibitors of MIF tautomerase activity.
Dios A
Journal of Medicinal Chemistry, 45(12), 2410-2416 (2002)
Simona Cardaropoli et al.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 34(7), 537-542 (2014-03-13)
Macrophage migration inhibitory factor (MIF) plays a pivotal role in pregnancy-related proinflammatory processes, such as placentation and labor. Differential MIF concentrations have been correlated with pathological events during pregnancy, such as recurrent miscarriages and severe pre-eclampsia (PE). The aim of
Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones.
McIntyre A
Oncogene, 23(56), 9142-9147 (2004)
Crystal J DiCosmo-Ponticello et al.
Cytokine, 69(1), 47-55 (2014-07-16)
Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that was initially identified by its ability to inhibit the movement of macrophages. Cell migration is a highly complex process involving changes to the cytoskeleton and cell adhesion molecules, and is

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