SRP3332

M-CSF rat

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): CSF-1, MGI-IM
• NACRES: NA.32
• UNSPSC Code: 12352202

Properties

• Product Name: M-CSF rat, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
• biological source: rat
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: ≤5.0 ng/mL ED₅₀
• mol wt: 36.2 kDa
• packaging: pkg of 10 μg
• impurities: tested
• UniProt accession no.: Q8JZQ0
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: rat ... CSF1(78965)

Description

Biochem/physiol Actions

Macrophage colony stimulating factor (M-CSF) is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility.[1][2] M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation.

General description

Macrophage colony stimulating factor (M-CSF) is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. The gene encoding this protein is localized on rat chromosome 2q34. The human protein is reactive in murine systems, but the murine molecule exhibits no activity on human cells.[1][3] Recombinant rat M-CSF is a 36.2kDa homodimeric protein containing two 155 amino acid polypeptide subunits.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable