SRP4178

IL-17A from rat

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(s): Ctla8, IL17, IL17a
• MDL number: MFCD01635117
• NACRES: NA.32

Properties

• biological source: rat
• recombinant: expressed in E. coli
• Assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 30 ng/mL
• mol wt: ~30 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: Q61453
• UniProt accession no.: Q61453
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: rat ... Il17a(25465)

Description

General description

IL-17A (interleukin 17A) is the first cytokine member of the family composed of IL-17A-F. These cytokines are mainly produced by activated CD4⁺ (cluster of differentiation) T cells. IL-17A is predominantly synthesized by T-helper (Th)-17 lymphocytes, γδ T cells (γδ-17), cytotoxic CD8⁺ T cells (Tc17), natural killer T (NKT) cells, and neutrophils.

Biochem/physiol Actions

IL-17A (interleukin 17A) acts as a regulator of immune responses in inflammatory disorders, and acts as a potent pleiotropic proinflammatory cytokine where it promotes the release of other pro-inflammatory cytokines from a variety of cells. This cytokine is linked with pain, and IL-17A and IL-17A⁺ T cells are found in patients with arthritic pain, and in induced animal models of sciatic nerve, and optic nerve injury with neuropathic pain. This protein is thought to be involved in myocardial remodeling and fibrosis, where it promotes the synthesis of collagens and matrix metalloproteinases (MMPs) and activates cardiomyocyte apoptosis.
Recombinant rat IL-17 produced from Escherichia coli is a homodimeric, non-glycosylated polypeptide containing 268 amino acids and has a molecular weight of 30kDa.

Physical form

Lyophilized (from 10 mM Sodium Citrate, pH 3.0).

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 μg/μL (Note: Gently pipetting to dissolve). The solution can then be diluted into other aqueous buffers.