IFN-alpha 1 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Interferon-α, IFNA1, IFN-α 1
MDL number:

biological source



expressed in E. coli


≥95% (HPLC)
≥95% (SDS-PAGE)




1x10^8 units/mg

mol wt

~19.3 kDa


pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles


endotoxin, tested

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.


Gene Information

human ... IFNA1(3439)

General description

IFN-α1 (interferon α 1) is a type 1 interferon. Human IFN-a1 is a 19.3kDa protein containing 166 amino acid residues. IFN-α1 belongs to the type I interferon (IFN) family and is present in leukocytes. The IFN-α1 gene is mapped to human chromosome 9p21.3.


IFN-alpha 1 human has been used:
  • to treat Vero and breast cancer (MDA-MB-231) cells in interferon assay before viral infection
  • as a ligand to vaccinia virus B18R protein in analytical size exclusion chromatography
  • to treat chikungunya infected human embryonic kidney cell line (HEK293T) to test its effect on Signal transducer and activator of transcription 1 (STAT1) phosphorylation
  • as recombinant interferon to treat lung fibroblast cell line (IMR90) to monitor histone chaperone (HIRA) response

Biochem/physiol Actions

IFN-α1 (interferon α 1) is a lymphoid factor that possesses potent anti-viral activity. IFN-α1 interacts with IFNAR1 (interferon α receptor 1) and IFNAR2. The interaction activates JAK1 (janus-activated kinase 1) and tyrosine kinase 2. These events phosphorylate and activate STAT1 and STAT2 (signal transducer and activator of transcription proteins), leading to the upregulation of interferon-stimulated genes some of which encode antiviral proteins. IFN-alpha 1 (IFN-α1) mediates apoptosis in specific tissues. It may be useful in treating laryngeal carcinoma. The interaction of microRNA, miR-181b with IFN-α1, is regarded as a risk factor in systemic lupus erythematosus.

Physical form

Lyophilized without any additives.


Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers.
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Yong-Ling He et al.
BioMed research international, 2020, 4757065-4757065 (2020-05-10)
A previous work has discovered that chromosome 1q32 locus linked to the risk of systemic lupus erythematosus (SLE) and miR-181b located on the susceptibility site with downregulation inversely correlating to its target molecular interferon alpha 1 (IFNA1). The purpose of...
K Oritani et al.
Cytokine & growth factor reviews, 12(4), 337-348 (2001-09-07)
The type I interferon (IFN) family includes IFN-alpha, IFN-beta, IFN-pi, and IFN-tau. These molecules are clustered according to sequence homologies, use of the same cell surface receptor, and similar functions. IFN-alpha and IFN-beta have a globular structure composed of five...
Saisai Wang et al.
Journal of cellular and molecular medicine, 19(10), 2432-2440 (2015-06-30)
Src homology phosphotyrosyl phosphatase 2 (Shp-2) is a ubiquitously expressed protein that is involved in a variety of cellular processes, including antiviral interferon signalling pathways. In this study, we investigated the role of Shp-2 in the host cell interactions of...
Yuriy G Kim et al.
PloS one, 12(12), e0189308-e0189308 (2017-12-08)
B18R protein of Vaccinia virus binds to type I interferons and inhibits activation of interferon-mediated signal transduction. Cells which have unimpaired interferon signaling such as primary cell cultures or some industrially important cell lines are capable of development of an...
Xiao-Lei Xin et al.
Medical science monitor : international medical journal of experimental and clinical research, 25, 7100-7114 (2019-09-23)
BACKGROUND Interferon alpha (IFNalpha) exerts its anti-proliferative effect on many human cancers. Among the 13 subtypes of human IFNalpha, IFNalpha-1 subtype has 2 variants, named IFNalpha-1a and IFNalpha-1b, that differ from each other in only 1 amino acid, at residue...

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