SRP4596

IFN-alpha 1 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(s): IFN-α 1, IFNA1, Interferon-α
• MDL number: MFCD01773209
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 1x10^8 units/mg
• mol wt: ~19.3 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: NM_024013
• UniProt accession no.: P01562
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IFNA1(3439)

Description

General description

IFN-α1 (interferon α 1) is a type 1 interferon.[1] Human IFN-a1 is a 19.3kDa protein containing 166 amino acid residues. IFN-α1 belongs to the type I interferon (IFN) family and is present in leukocytes.[2] The IFN-α1 gene is mapped to human chromosome 9p21.3.[3]

Application

IFN-alpha 1 human has been used:

• to treat Vero and breast cancer (MDA-MB-231) cells in interferon assay before viral infection[4]
• as a ligand to vaccinia virus B18R protein in analytical size exclusion chromatography[5]
• to treat chikungunya infected human embryonic kidney cell line (HEK293T) to test its effect on Signal transducer and activator of transcription 1 (STAT1) phosphorylation[6]
• as recombinant interferon to treat lung fibroblast cell line (IMR90) to monitor histone chaperone (HIRA) response[7]

Biochem/physiol Actions

IFN-α1 (interferon α 1) is a lymphoid factor that possesses potent anti-viral activity.[8] IFN-α1 interacts with IFNAR1 (interferon α receptor 1) and IFNAR2. The interaction activates JAK1 (janus-activated kinase 1) and tyrosine kinase 2. These events phosphorylate and activate STAT1 and STAT2 (signal transducer and activator of transcription proteins), leading to the upregulation of interferon-stimulated genes some of which encode antiviral proteins.[9] IFN-alpha 1 (IFN-α1) mediates apoptosis in specific tissues. It may be useful in treating laryngeal carcinoma.[10] The interaction of microRNA, miR-181b with IFN-α1, is regarded as a risk factor in systemic lupus erythematosus.[11]

Physical form

Lyophilized without any additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable