SRP4639

BMP-13 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(s): CDMP-2, Cartilage-Derived Morphogenetic Protein-2, GDF-6, bone morphogenetic protein 13
• NACRES: NA.32
• UNSPSC Code: 12352202

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 0.5-4 μg/mL ED₅₀ (determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells.)
• mol wt: ~27 kDa
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: Q6KF10
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... GDF6(392255)

Description

General description

BMPs (bone morphogenetic proteins) belong to the TGF-β superfamily of structurally related signaling proteins. These ligands mediate numerous physiological processes. BMPs initiate, promote and maintain cartilage and bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. Expression of BMP-13 has been found in the hypertrophic chondrocytes of ossifying long bone centers. Recombinant human BMP-13/CDMP-2 is a 27 kDa homodimeric disulfide-linked protein consisting of two 120 amino acids polypeptide chains.

Physical form

Sterile filtered and Lyophilized without additives.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute to 0.1-1.0 mg/mL in water containing BSA (50 μg BSA per 1 μg of protein).