SRP4754

Sigma-Aldrich

WNT-1 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):
INT-1, WNT1
MDL number:
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.5-2.5 ng/mL ED50

mol wt

~38.4 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... WNT1(7471)

General description

Wnt family member 1 (Wnt-1) is a secreted protein that signals through the Frizzled family of cell surface receptors. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. The gene encoding this protein is localized on human chromosome 12q13.12. Recombinant human Wnt-1 is a 38.4kDa, non-glycosylated protein containing 343 amino acid residues.

Application

Wnt family member 1 (Wnt-1) has been used in the differentiation medium for human neural progenitor cells (hNP1).

Biochem/physiol Actions

Wnt family member 1 (Wnt-1) and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over 50 genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers.

Physical form

Sterile filtered and lyophilized with no additives.

Reconstitution

Reconstitute in water to a concentration of 0.1-1.0 μg/μL. The solution can then be diluted into other aqueous buffers.
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1.
Guo X
Biomaterials, 34(18), 4418-4427 (2013)
Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions.
Cai L
Cell Research, 17(1), 62-72 (2007)
Wnt/beta-catenin signaling: components, mechanisms, and diseases.
MacDonald BT
Developmental Cell, 17(1), 9-26 (2009)
Cross-talk of WNT and FGF signaling pathways at GSK3beta to regulate beta-catenin and SNAIL signaling cascades.
Katoh M and Katoh M
Cancer Biology & Therapy, 5(9), 1059-1064 (2006)

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