SRP4896

TSLP human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)

• Synonym(s): Thymic Stromal Lymphopoietin
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥97% (HPLC); ≥97% (SDS-PAGE)
• form: lyophilized
• potency: <0.3 ng/mL ED₅₀
• mol wt: ~15.0 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: Q969D9
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TSLP(85480)

Description

General description

Thymic stromal lymphopoietin (TSLP) is a hemopoietic cytokine. It is part of the interleukin-2 (IL-2) cytokine family. It is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities to interleukin-7 (IL-7) and binds with the heterodimeric receptor complex consisting of interleukin-7 receptor α chain & the TSLP receptor. Recombinant human TSLP produced in Escherichia coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15kDa.

Application

TSLP human has been used in immunohistochemistry.

Biochem/physiol Actions

Thymic stromal lymphopoietin (TSLP) signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the interleukin-7 (IL-7) receptor a chain. TSLP impacts myeloid cells and thus induces the discharge of T cell-attracting chemokines from monocytes and increases the growth of CD11c⁺ dendritic cells. Similar to IL-7, TSLP enhances phosphorylation of signal transducer and activator of transcription 3 and 5 (STAT3 and STAT5), though uses kinases excluding Janus kinases (JAKs) for its activation. TSLP induces the release of T cell-attracting chemokines such as thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) from monocytes and triggers CD11c⁺ dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as interleukin-4, interleukin-5, interleukin-13 and tumor necrosis factor-α (TNF-α), whereas down-regulating interleukin-10 and interferon-γ (IFN-γ) play a role in the initiation of allergic inflammation.

Physical form

Lyophilized from a concentrated solution (1 mg/ml) containing 130 mM NaCl, and 20 mM sodium phosphate, pH 7.4.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in sterile dH₂O to a concentration of 0.1 -1 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable