We do not see any evidence of a tag for SRP4896. Furthermore, TSLP protein is a hemopoietic cytokine that signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor & the Interleukin-7 receptor alpha chain. TSLP impacts myeloid cells, thus inducing the discharge of T cell-attracting chemokines from monocytes & increasing the growth of CD11c(+) dendritic cells. TSLP is mainly expressed in the heart, liver, and prostate. TSLP is related in its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the Interleukin-7 receptor alpha chain & the TSLPR. Similar to IL-7, TSLP enhances phosphorylation of STAT3 and STAT5, though it uses kinases excluding JAKs for its activation. TSLP induces the release of T cell-attracting chemokines such as TARC & MDC from monocytes & triggers CD11c(+) dendritic cells. Recombinant human TSLP produced in E. Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa.
SRP4896
TSLP human
recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)
Synonym(s):
Thymic Stromal Lymphopoietin
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biological source
human
recombinant
expressed in E. coli
assay
≥97% (HPLC), ≥97% (SDS-PAGE)
form
lyophilized
potency
<0.3 ng/mL ED50
mol wt
~15.0 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TSLP(85480)
1 of 4
This Item | SRP8032 | SRP3060 | SRP4193 |
|---|---|---|---|
| assay ≥97% (HPLC), ≥97% (SDS-PAGE) | assay >95% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) |
| recombinant expressed in E. coli | recombinant expressed in HEK 293 cells | recombinant expressed in E. coli | recombinant expressed in E. coli |
| biological source human | biological source human | biological source human | biological source human |
| form lyophilized | form lyophilized | form lyophilized | form lyophilized |
| Gene Information human ... TSLP(85480) | Gene Information human ... IL1R1(3554) | Gene Information human ... IL28A(282616) | Gene Information human ... IL33(90865) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
Thymic stromal lymphopoietin (TSLP) is a hemopoietic cytokine. It is part of the interleukin-2 (IL-2) cytokine family. It is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities to interleukin-7 (IL-7) and binds with the heterodimeric receptor complex consisting of interleukin-7 receptor α chain & the TSLP receptor.[1] Recombinant human TSLP produced in Escherichia coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15kDa.
Application
TSLP human has been used in immunohistochemistry.[2]
Biochem/physiol Actions
Thymic stromal lymphopoietin (TSLP) signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the interleukin-7 (IL-7) receptor a chain. TSLP impacts myeloid cells and thus induces the discharge of T cell-attracting chemokines from monocytes and increases the growth of CD11c+ dendritic cells. Similar to IL-7, TSLP enhances phosphorylation of signal transducer and activator of transcription 3 and 5 (STAT3 and STAT5), though uses kinases excluding Janus kinases (JAKs) for its activation. TSLP induces the release of T cell-attracting chemokines such as thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) from monocytes and triggers CD11c+ dendritic cells.[3] TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as interleukin-4, interleukin-5, interleukin-13 and tumor necrosis factor-α (TNF-α), whereas down-regulating interleukin-10 and interferon-γ (IFN-γ) play a role in the initiation of allergic inflammation.[1]
Physical form
Lyophilized from a concentrated solution (1 mg/ml) containing 130 mM NaCl, and 20 mM sodium phosphate, pH 7.4.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in sterile dH2O to a concentration of 0.1 -1 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Thymic stromal lymphopoietin expression is increased in asthmatic airways and correlates with expression of Th2-attracting chemokines and disease severity.
Ying S
Journal of Immunology, 174(12), 8183-8190 (2005)
The biology of thymic stromal lymphopoietin (TSLP).
Ziegler SF
Advances in Pharmacology, 66, 129-155 (2013)
Unusual Timing of CD127 Expression by Mouse Uterine Natural Killer Cells
Jianhong Zhang
Journal of Leukocyte Biology, 91(3), 417-426 (2012)
Chun I Yu et al.
Journal of immunology (Baltimore, Md. : 1950), 193(9), 4335-4343 (2014-09-24)
Dendritic cells (DCs) play the central role in the priming of naive T cells and the differentiation of unique effector T cells. In this study, using lung tissues and blood from both humans and humanized mice, we analyzed the response
Frederique M Moret et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(5), 1176-1184 (2014-05-02)
To determine the levels of thymic stromal lymphopoietin (TSLP) and the numbers of TSLP receptor (TSLPR)-expressing CD1c+ (blood dendritic cell antigen 1-positive) myeloid dendritic cells (MDCs) in the joints as compared with the peripheral blood (PB) of patients with rheumatoid
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Is there any tag in n-or c- terminal of this protein?
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