SRP4896

Sigma-Aldrich

TSLP human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)

Synonym(s):
Thymic Stromal Lymphopoietin
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥97% (HPLC)
≥97% (SDS-PAGE)

form

lyophilized

potency

<0.3 ng/mL ED50

mol wt

~15.0 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TSLP(85480)

General description

Thymic stromal lymphopoietin (TSLP) is a hemopoietic cytokine. It is part of the interleukin-2 (IL-2) cytokine family. It is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities to interleukin-7 (IL-7) and binds with the heterodimeric receptor complex consisting of interleukin-7 receptor α chain & the TSLP receptor. Recombinant human TSLP produced in Escherichia coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15kDa.

Application

TSLP human has been used in immunohistochemistry.

Biochem/physiol Actions

Thymic stromal lymphopoietin (TSLP) signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the interleukin-7 (IL-7) receptor a chain. TSLP impacts myeloid cells and thus induces the discharge of T cell-attracting chemokines from monocytes and increases the growth of CD11c+ dendritic cells. Similar to IL-7, TSLP enhances phosphorylation of signal transducer and activator of transcription 3 and 5 (STAT3 and STAT5), though uses kinases excluding Janus kinases (JAKs) for its activation. TSLP induces the release of T cell-attracting chemokines such as thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) from monocytes and triggers CD11c+ dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as interleukin-4, interleukin-5, interleukin-13 and tumor necrosis factor-α (TNF-α), whereas down-regulating interleukin-10 and interferon-γ (IFN-γ) play a role in the initiation of allergic inflammation.

Physical form

Lyophilized from a concentrated solution (1 mg/ml) containing 130 mM NaCl, and 20 mM sodium phosphate, pH 7.4.

Reconstitution

Reconstitute in sterile dH2O to a concentration of 0.1 -1 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers.
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Thymic stromal lymphopoietin expression is increased in asthmatic airways and correlates with expression of Th2-attracting chemokines and disease severity.
Ying S
Journal of Immunology, 174(12), 8183-8190 (2005)
Unusual Timing of CD127 Expression by Mouse Uterine Natural Killer Cells
Jianhong Zhang
Journal of Leukocyte Biology, 91(3), 417-426 (2012)
The biology of thymic stromal lymphopoietin (TSLP).
Ziegler SF
Advances in Pharmacology, 66, 129-155 (2013)
Frederique M Moret et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(5), 1176-1184 (2014-05-02)
To determine the levels of thymic stromal lymphopoietin (TSLP) and the numbers of TSLP receptor (TSLPR)-expressing CD1c+ (blood dendritic cell antigen 1-positive) myeloid dendritic cells (MDCs) in the joints as compared with the peripheral blood (PB) of patients with rheumatoid...
Chun I Yu et al.
Journal of immunology (Baltimore, Md. : 1950), 193(9), 4335-4343 (2014-09-24)
Dendritic cells (DCs) play the central role in the priming of naive T cells and the differentiation of unique effector T cells. In this study, using lung tissues and blood from both humans and humanized mice, we analyzed the response...

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