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SRP4975

Sigma-Aldrich

IL-24 human

recombinant, expressed in Saccharomyces cerevisiae, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):
C49A, FISP, IL-24, IL10B, MDA7, ST16
NACRES:
NA.32

biological source

human

recombinant

expressed in Saccharomyces cerevisiae

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

~19.5 kDa

packaging

pkg of 10 μg

impurities

endotoxin, tested

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL24(11009)

General description

IL24 is a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. IL-10B encoded can induce apoptosis selectively in various cancer cells. Overexpression IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. The glycosylation is essential for activity of IL-24. Functionally, IL-24 has diverse activities. At low concentrations, it induces type I proinflammatory cytokines such as IFN-γ, IL-1β, IL-12 and TNF-α. At high concentration, it is a strong inducer of apoptosis in tumor cells, but not normal cells. mda-7/IL-24 is being hailed as a ‘magic bullet′ for cancer gene therapy. Recombinant human Il-24 produced in yeast is a single, glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 18 kDa. As a result of glycosilation, the protein migrates at 19.5 kDa on SDS-PAGE.

Physical form

Lyophilized from PBS with BSA as a carrier.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in sterile dH2O not less than 100 μg/ml. This solution can then be diluted into other aqueous buffers.

Certificate of Analysis

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Certificate of Origin

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