SRP5003

AMPK (α2/β2/γ1), active, His tagged human

Name: AMPK (α2/β2/γ1), active, His tagged human
Brand: SIGMA
Price: 735 USD

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

AMPK, AMPK2, AMPKG, MGC61468, MGC8666, PRKAA, PRKAA2, PRKAB2, PRKAG1

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5 μG

$735.00

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.32

Key Documents

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recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

238-322 nmol/min·mg

mol wt

~36 kDa (B2)
~40 kDa (G1)
~69 kDa (A2)

NCBI accession no.

NM_002733
NM_005399
NM_006252

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKAA2(5563), PRKAB2(5565), PRKAG1(5571)

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This Item	SRP5004	A4611	A4486
Sigma-Aldrich SRP5003 AMPK (α2/β2/γ1), active, His tagged human	Sigma-Aldrich SRP5004 AMPK (α2/β2/γ2), active, His tagged human	Sigma-Aldrich A4611 AMPK (α1/β2/γ1), active, His tagged human	Sigma-Aldrich A4486 AMPK (α1/β1/γ3), active, His tagged human
assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)
Gene Information human ... PRKAA2(5563), PRKAB2(5565), PRKAG1(5571)	Gene Information human ... PRKAA2(5563), PRKAB2(5565), PRKAG2(51422)	Gene Information human ... PRKAA1(5562), PRKAB2(5565), PRKAG1(5571)	Gene Information human ... PRKAA1(5562), PRKAB1(5564), PRKAG3(53632)
specific activity 238-322 nmol/min·mg	specific activity 255-345 nmol/min·mg	specific activity 297-401 nmol/min·mg	specific activity 157-213 nmol/min·mg
recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
mol wt ~36 kDa (B2), ~69 kDa (A2), ~40 kDa (G1)	mol wt ~36 kDa (B2), ~65 kDa (G2), ~69 kDa (A2)	mol wt A1 subunit ~68 kDa, B2 subunit ~36 kDa, G1 subunit ~40 kDa	mol wt A1 subunit ~68 kDa, B1 subunit ~38 kDa, G3 subunit ~51 kDa

General description

AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H360D

pcodes

P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

R100-2

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PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia.

Qin Zhao et al.

Molecular cell, 77(1), 95-107 (2019-10-20)

GTP cyclohydrolase I (GTPCH), 6-pyruvoyltetrahydropterin synthase (PTPS), and sepiapterin reductase (SR) are sequentially responsible for de novo synthesis of tetrahydrobiopterin (BH4), a known co-factor for nitric oxide synthase (NOS). The implication of BH4-biosynthesis process in tumorigenesis remains to be investigated.

AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism.

Barbara B Kahn et al.

Cell metabolism, 1(1), 15-25 (2005-08-02)

The AMP-activated protein kinase (AMPK) is an evolutionarily conserved sensor of cellular energy status, and recent data demonstrate that it also plays a critical role in systemic energy balance. AMPK integrates nutritional and hormonal signals in peripheral tissues and the

O-GlcNAcylation of fumarase maintains tumour growth under glucose deficiency.

Ting Wang et al.

Nature cell biology, 19(7), 833-843 (2017-06-20)

Chromatin-associated fumarase (FH) affects histone methylation via its metabolic activity. However, whether this effect is involved in gene transcription remains to be clarified. In this study, we show that under glucose deprivation conditions, AMPK phosphorylates FH at Ser75, which in

Acetylation-dependent glutamate receptor GluR signalosome formation for STAT3 activation in both transcriptional and metabolism regulation.

Xiang-Rong Li et al.

Cell death discovery, 7(1), 11-11 (2021-01-16)

Besides their original regulating roles in the brain, spinal cord, retina, and peripheral nervous system for mediating fast excitatory synaptic transmission, glutamate receptors consisting of metabotropic glutamate receptors (GluRs) and ionotropic glutamate receptors (iGluRs) have emerged to have a critical

The AMP-activated protein kinase pathway--new players upstream and downstream.

D Grahame Hardie

Journal of cell science, 117(Pt 23), 5479-5487 (2004-10-29)

The AMP-activated protein kinase (AMPK) cascade is a sensor of cellular energy status. Whenever the cellular ATP:ADP ratio falls, owing to a stress that inhibits ATP production or increases ATP consumption, this is amplified by adenylate kinase into a much

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