SRP5051

NEK11, active, GST tagged human

Name: NEK11, active, GST tagged human
Brand: SIGMA
Price: 430 USD

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

FLJ23495

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10 μG

$430.00

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.32

Key Documents

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recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

21-29 nmol/min·mg

mol wt

~110 kDa

NCBI accession no.

NM_024800

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... NEK11(79858)

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This Item	SAB1407982	SRP5240	SRP5086
Sigma-Aldrich SRP5051 NEK11, active, GST tagged human	Sigma-Aldrich SAB1407982 Anti-NEK11 antibody produced in mouse	Sigma-Aldrich SRP5240 TLK1, active, GST tagged human	Sigma-Aldrich SRP5086 SLK, active, GST tagged human
assay ≥70% (SDS-PAGE)	assay -	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)
recombinant expressed in baculovirus infected Sf9 cells	recombinant -	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
mol wt ~110 kDa	mol wt antigen ~55.5 kDa	mol wt ~135 kDa	mol wt ~180 kDa
form buffered aqueous glycerol solution	form buffered aqueous solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
specific activity 21-29 nmol/min·mg	specific activity -	specific activity 5.3-7.1 nmol/min·mg	specific activity 3.9-5.3 nmol/min·mg
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

General description

NEK11 is a member of the NEK family and the protein localizes to the nucleoli where it may function with NEK2A in the S-phase checkpoint. NEK2 phosphorylates NEK11 in its C-terminal autoinhibitory domain and elevates NEK11 kinase activity by dissociating the autoinhibitory domain from the N-terminal catalytic domain. NEK11 protein appears to play a role in DNA replication and response to genotoxic stress. NEK11 activity is activated in HeLa cells treated with various DNA-damaging agents and replication inhibitors. Activation of NEK11 by genotoxic agents is suppressed by inhibitor of ATR/ATM-mediated signalling indicating that NEK11 has a role in S-phase checkpoint downstream of ATR/ATM signalling.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

W152-3

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Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses.

Kohji Noguchi et al.

The Journal of biological chemistry, 277(42), 39655-39665 (2002-08-03)

DNA replication and genotoxic stresses activate various checkpoint-associated protein kinases, and checkpoint dysfunction often leads to cell lethality. Here, we have identified new members of the mammalian NIMA family of kinases, termed Nek11L and Nek11S (NIMA-related kinase 11 Long and

Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells.

Kohji Noguchi et al.

The Journal of biological chemistry, 279(31), 32716-32727 (2004-05-27)

We previously reported that Nek11, a member of the NIMA (never-in-mitosis A) family of kinases, is activated in G(1)/S-arrested cells. We provide herein several lines of evidence for a novel interaction between Nek11 and Nek2A. Both Nek11 and Nek2A, but

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NEK11, active, GST tagged human