SRP5086

SLK, active, GST tagged human

Name: SLK, active, GST tagged human
Brand: SIGMA
Price: 587 USD

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

KIAA0204, LOSK, MGC133067, STK2, bA16H23.1, se20-9

Slide 1 of 2

Select a Size

10 μG

$587.00

Usually ships in 2 weeks.

About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

Form:

buffered aqueous glycerol solution

Assay:

≥70% (SDS-PAGE)

Recombinant:

expressed in baculovirus infected Sf9 cells

Mol wt:

~180 kDa

Key Documents

View All Documentation

Product Name

SLK, active, GST tagged human, PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

3.9-5.3 nmol/min·mg

mol wt

~180 kDa

NCBI accession no.

AB002804

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SLK(9748)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	SRP5285	SRP5369	SRP5328
Sigma-Aldrich SRP5086 SLK, active, GST tagged human	Sigma-Aldrich SRP5285 NLK, active, GST tagged human	Sigma-Aldrich SRP5369 VLK, GST tagged human	Sigma-Aldrich SRP5328 NEK5, active, GST tagged human
assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)
recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
mol wt ~180 kDa	mol wt ~84 kDa	mol wt ~69 kDa	mol wt ~84 kDa
form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
storage temp. −70°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C
specific activity 3.9-5.3 nmol/min·mg	specific activity 1.9-2.9 nmol/min·mg	specific activity -	specific activity 80-120 nmol/min·mg

General description

SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement indicating that SLK is needed for microtubule organization and for the proper localization of Golgi complex. Expression of v-Src can down-regulate SLK activity through the involvement of CK2 which can directly phosphorylate and inhibit SLK activity whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity. CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

H307-2

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

LOSK (SLK) protein kinase activity is necessary for microtubule organization in the interphase cell centrosome.

A V Burakov et al.

Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 403, 317-319 (2005-12-20)

v-Src-dependent down-regulation of the Ste20-like kinase SLK by casein kinase II.

Ziad Chaar et al.

The Journal of biological chemistry, 281(38), 28193-28199 (2006-07-14)

We have previously shown that the Ste20-like kinase SLK is a microtubule-associated protein inducing actin stress fiber disassembly. Here, we show that v-Src expression can down-regulate SLK activity. This down-regulation is independent of focal adhesion kinase but requires v-Src kinase

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

SLK, active, GST tagged human