SRP5171

Catenin α, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): CAP102, CTNNA1, FLJ36832
• CAS Number: 847964-75-4
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~123 kDa
• NCBI accession no.: NM_001903
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CTNNA1(1495)

Description

General description

Catenin α is a novel actin-binding and -bundling protein. Catenin α is responsible for organizing and tethering actin filaments at the zones of E-cadherin-mediated cell-cell contact. Monomeric Catenin α can bind strongly to E-Cadherin-β-Catenin, whereas the dimer preferentially binds actin filaments. Different molecular conformations are associated with these different binding states, indicating that Catenin α is an allosteric protein. Catenin α directly regulates actin-filament organization by suppressing Arp2/3-mediated actin polymerization, likely by competing with the Arp2/3 complex for binding to actin filaments

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1