SRP5240

TLK1, active, GST tagged human

Name: TLK1, active, GST tagged human
Brand: SIGMA
Price: 587 USD

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

KIAA0137, PKU-β

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10 μG

$587.00

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

Form:

buffered aqueous glycerol solution

Assay:

≥70% (SDS-PAGE)

Recombinant:

expressed in baculovirus infected Sf9 cells

Mol wt:

~135 kDa

Key Documents

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Product Name

TLK1, active, GST tagged human, PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

5.3-7.1 nmol/min·mg

mol wt

~135 kDa

NCBI accession no.

NM_012290

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TLK1(9874)

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1 of 4

This Item	SRP5051	SRP5356	SRP5093
Sigma-Aldrich SRP5240 TLK1, active, GST tagged human	Sigma-Aldrich SRP5051 NEK11, active, GST tagged human	Sigma-Aldrich SRP5356 OSR1, GST tagged human	Sigma-Aldrich SRP5093 TLK2 (388-end), active, GST tagged human
assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥80% (SDS-PAGE)
recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
mol wt ~135 kDa	mol wt ~110 kDa	mol wt ~86 kDa	mol wt ~67 kDa
form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
storage temp. −70°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C
Gene Information human ... TLK1(9874)	Gene Information human ... NEK11(79858)	Gene Information human ... OXSR1(9943)	Gene Information human ... TLK2(11011)

General description

TLK1 or tousled-like kinase 1 is a nuclear serine/threonine kinase that is potentially involved in the regulation of chromatin assembly. TLK1 is first described in Arabidopsis. TLK1 is a novel target of the DNA damage checkpoint that is rapidly inactivated upon exposure to ionizing radiation (IR) and the inactivation is directly mediated by the S-phase DNA damage checkpoint. The suppression of TLK1 activity after double-strand breaks in the DNA, replication blockade, or low doses of ultraviolet irradiation requires ATM, NBS and CHK1.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number

I351-2

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Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1.

Darren R Krause et al.

Oncogene, 22(38), 5927-5937 (2003-09-05)

The human Tousled-like kinases 1 and 2 (TLK) have been shown to be active during S phase of the cell cycle. TLK activity is rapidly suppressed by DNA damage and by inhibitors of replication. Here we report that the signal

Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint.

Anja Groth et al.

The EMBO journal, 22(7), 1676-1687 (2003-03-28)

All eukaryotes respond to DNA damage by modulation of diverse cellular processes to preserve genomic integrity and ensure survival. Here we identify mammalian Tousled like kinases (Tlks) as a novel target of the DNA damage checkpoint. During S-phase progression, when

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TLK1, active, GST tagged human