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SRP5246

Sigma-Aldrich

PAK1, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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Synonym(s):
MGC130000, MGC130001, PAKα
CAS Number:
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

63-85 nmol/min·mg

mol wt

~95 kDa

NCBI accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PAK1(5058)

General description

p21 (RAC1) activated kinase 1 (PAK1) is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3. The gene encoding this protein is localized on human chromosome 11q13.

Biochem/physiol Actions

p21-activated kinases (PAKs) are serine/threonine kinases that are activated by and are effectors of small GTPases, cell division control protein 42 (CDC42) and Ras-related C3 botulinum toxin substrate (RAC). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix. The protein has a role in numerous cellular processes and has been shown to be expressed in squamous non-small cell lung carcinomas.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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