SRP5328

NEK5, active, GST tagged human

PRECISIO^®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): MGC75495
• CAS Number: 2249882-56-0
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• product line: PRECISIO^®
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 80-120 nmol/min·mg
• mol wt: ~84 kDa
• NCBI accession no.: NM_199289
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... NEK5(341676)

Description

General description

NEK5 or NIMA (never in mitosis gene a)-related kinase 5 is a serine/threonine kinase that is involved in cell cycle regulation. NEK5 has been shown to interact with NEK6 and NEK5 are required for the ibo1/nek6 ectopic outgrowth phenotype in epidermal cells. NEK6 interacts and co-localizes with NEK5 in a transient expression assay. The ibo1-3 mutation markedly increases the interaction between NEK6 and NEK5. NEK6 forms heterodimers with NEK5 to regulate cortical microtubule organization possibly through the phosphorylation of β-tubulins. The NEK5 gene is conserved in chimpanzee, cow, mouse, rat, and chicken.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable