SRP6045

Glucokinase from human liver

recombinant, expressed in E. coli, ≥77% (SDS-PAGE)

• Synonym(s): Hexokinase, Hexokinase, liver, Liver glucokinase
• CAS Number: 9001-51-8
• NACRES: NA.32
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• Form: liquid
• Assay: ≥77% (SDS-PAGE)
• Biological source: human liver
• Recombinant: expressed in E. coli
• Mol wt: 53.4 kDa

Properties

• biological source: human liver
• recombinant: expressed in E. coli
• assay: ≥77% (SDS-PAGE)
• form: liquid
• mol wt: 53.4 kDa
• packaging: pkg of 10 μg
• concentration: ~1 mg/mL
• impurities: Endotoxin, tested
• NCBI accession no.: P35557
• UniProt accession no.: P35557
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... GCK(2645)

Description

General description

The gene encoding glucokinase (GCK) is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The enzyme is expressed as a monomer and possesses one glucose-binding site. The gene encoding GCK is localized on human chromosome 7. Recombinant human liver glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues.

Biochem/physiol Actions

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. The main function of glucokinase (GCK) is to regulate carbohydrate metabolism. The enzyme triggers the release of insulin in β-cells of pancreas. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity. Mutation in the gene encoding this protein is associated with a rare form of diabetes and the protein may also have a role in type 2 diabetes.

Physical form

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.

Other Notes

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30°C for 30 min.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable