MilliporeSigma
All Photos(1)

Documents

SRP6087

Sigma-Aldrich

AKR1C1 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

All Photos(1)
Synonym(s):
20-alpha HSD, Aldo-keto reductase family 1 member C1, DD1/DD2, DDH1, HBAB
CAS Number:
1527538-64-2
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

liquid

mol wt

38.9 kDa

packaging

pkg of 100 μg

NCBI accession no.

Q04828

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... AKR1C1(1645)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SRP6089SRP6371SRP6090
AKR1C1 human recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Sigma-Aldrich

SRP6087

AKR1C1 human

AKR1C4 human recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Sigma-Aldrich

SRP6089

AKR1C4 human

Aldose Reductase human recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Sigma-Aldrich

SRP6371

Aldose Reductase human

AKR1B10 human recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Sigma-Aldrich

SRP6090

AKR1B10 human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

form

liquid

form

liquid

form

liquid

form

liquid

mol wt

38.9 kDa

mol wt

39.2 kDa

mol wt

35.8 kDa (316 aa, 1-316 aa)

mol wt

36 kDa

packaging

pkg of 100 μg

packaging

pkg of 100 μg

packaging

pkg of 100 μg

packaging

pkg of 100 μg

General description

AKR1C1 (aldo-keto reductase 1C1) is one of the four isoforms (AKR1C1–AKR1C4) of the phase I drug-metabolizing enzyme family called AKR1C. This enzyme is thought to be composed of 323 amino acids, and its mRNA is expressed in brain, mammary gland tissues, liver, adrenal, prostate, uterus and testis.

Biochem/physiol Actions

AKR1C (aldo-keto reductase 1C) family of proteins is responsible for maintaining steroid homeostasis, activation of polycyclic aromatic hydrocarbons and the metabolism of prostaglandin. These enzymes function as NAD(P)(H)-dependent oxidoreductases and are responsible for the production of alcohol by catalyzing the reduction of aldehydes and ketones. Studies show that up-regulation of AKR1C1 is linked with resistance to anti-cancer therapeutics such as, adriamycin, daunorubicin and cisplatin. In human endometrial tissues, this protein is responsible for the conversion of progesterone to a biologically inactive metabolite. Studies in cultured endometrial stromal cells show that the mRNA expression level of AKR1C1 is inversely dependent on the level of progesterone, thus suggesting that progesterone controls its own local concentration through AKR1C1 enzyme in endometrial stromal cells at peri-implantation periods.

Physical form

0.5 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 20% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Other Notes

MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Characterization of a human 20alpha-hydroxysteroid dehydrogenase.
Zhang Y, et al.
Journal of Molecular Endocrinology, 25(2), 221-228 (2000)
Human cytosolic hydroxysteroid dehydrogenases of the aldo-ketoreductase superfamily catalyze reduction of conjugated steroids: implications for phase I and phase II steroid hormone metabolism.
Jin Y, et al.
The Journal of Biological Chemistry, 284(15), 10013-10022 (2009)
Induction of neoplastic transformation by ectopic expression of human aldo-keto reductase 1C isoforms in NIH3T3 cells.
Chien CW, et al.
Carcinogenesis, 30(10), 1813-1820 (2009)
Expression of 20alpha-hydroxysteroid dehydrogenase mRNA in human endometrium and decidua.
Nakajima T, et al.
Endocrine Journal, 50(1), 105-111 (2003)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service