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SRP6112

Sigma-Aldrich

G6PD from E. coli

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):
Glucose-6-phosphate 1-dehydrogenase, Zwf
CAS Number:
NACRES:
NA.32

biological source

Escherichia coli

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

liquid

glucose-6-phosphate dehydrogenase activity

8-10 U/mL (Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP.)

mol wt

55.7 kDa (491 aa, 1-491 aa)

packaging

pkg of 100 μg

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

Escherichia coli ... ZWF(7149716)

General description

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produces NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency causes acute hemolytic anemia.

Physical form

1 mg/mL solution in 50 mM MES pH 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT and 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Other Notes

MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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