SRP6147

Sigma-Aldrich

Ubiquitin Activating Enzyme E1 human

≥95% (SDS-PAGE)

Synonym(s):
UBA1, POC20, UBE1, AMCX1, Ubiquitin E1
NACRES:
NA.32

biological source

human

assay

≥95% (SDS-PAGE)

form

liquid

potency

>3 nM

mol wt

118

packaging

pkg of 50 μg

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UBA1(7317)

General description

Ubiquitin activating enzyme 1 (UBA1) or UBE1, is an ubiquitin-activating enzyme. The gene encoding it is localized on human chromosome Xp11.23.

Biochem/physiol Actions

Ubiquitin activating enzyme 1 (UBA1) has a role in cellular protein homeostasis. It is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate. The protein has been associated with spinal muscular atrophy (SMA) and Huntington′s disease (HD).

Physical form

In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

E2 enzymes: more than just middle men.
Stewart MD
Cell Research, 26(4), 423-440 (2016)
Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer.
Olsen SK and Lima CD
Molecular Cell, 49(5), 884-896 (2013)
UBA1: At the Crossroads of Ubiquitin Homeostasis and Neurodegeneration.
Groen EJ and Gillingwater TH
Trends in Molecular Medicine, 21(10), 622-632 (2015)
UBE1 expression in extranodal NK/T cell lymphoma, nasal type.
Z L Su et al.
Leukemia & lymphoma, 49(9), 1821-1822 (2008-07-29)

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