SRP6158

EPO human

Name: EPO human
Brand: SIGMA

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

EP, EPO-alpha, Epoetin, Erythropoietin-alpha, MGC138142

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About This Item

CAS Number:

11096-26-7

UNSPSC Code:

12352202

NACRES:

NA.32

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Key Documents

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biological source

human

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

36 kDa (monomer, glycosylated)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

NCBI accession no.

P01588

UniProt accession no.

P01588

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EPO(2056)

Related Categories

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Growth Factors and Cytokines

Molecular Biology & Functional Genomics

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Show Differences

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This Item	E9530	H5791	H7041
Sigma-Aldrich SRP6158 EPO human	Sigma-Aldrich E9530 Erythropoietin from mouse	Sigma-Aldrich H5791 Hepatocyte Growth Factor human	Sigma-Aldrich H7041 Interleukin-2 human
biological source human	biological source mouse	biological source human	biological source human
recombinant expressed in HEK 293 cells	recombinant expressed in NSO cells	recombinant expressed in HEK 293 cells	recombinant expressed in HEK 293 cells
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
assay ≥95% (SDS-PAGE)	assay ≥90% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay -
form lyophilized	form lyophilized powder	form lyophilized powder	form lyophilized powder
mol wt 36 kDa (monomer, glycosylated)	mol wt ~36 kDa by SDS-PAGE, predicted mol wt 18.6 kDa	mol wt monomer 70 kDa (glycosylated)	mol wt monomer 15 kDa (glycosylated)

General description

The gene EPO (erythropoietin) is mapped to human chromosome 7q22.[1] It is a glycoprotein hormone. EPO is generated in fetal liver and adult kidney. However, it can also be secreted by other organs, such as the heart, brain and lungs.[2] EPO is a member of the EPO/TPO (thrombopoietin) family and is found in the plasma.[3]

Application

EPO (erythropoietin) has been used as a component of the culture medium for cardiac stem cells.[4]
In cultured cerebellar granule cells and hippocampal neurons, it has been used to study effect of EPO on glutamate release.[5]
It has also been used as a component of endothelial differentiation medium.[6]

Biochem/physiol Actions

EPO (erythropoietin) regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.[3] It interacts with EPO receptor and is responsible for the cellular responses. EPO is expressed in many cancers, including breast cancer, renal cancer, gastric cancer, hepatocellular carcinoma and central nervous system tumors. In addition, it is a hypoxia responsive cytokine and a pro-angiogenic factor.[2] This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.[7] It can protect from brain damages due to ischemia and enhances memory as well as cognitive ability in humans suffering from vascular dementia.[7]

Physical form

Lyophilized from a PBS solution.

Preparation Note

Centrifuge the vial prior to opening.

Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

3H11L64470

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Equine performance genes and the future of doping in horseracing.

Tessa Wilkin et al.

Drug testing and analysis, 9(9), 1456-1471 (2017-03-30)

A horse's success on the racetrack is determined by genetics, training and nutrition, and their translation into physical traits such as speed, endurance and muscle strength. Advances in genetic technologies are slowly explaining the roles of specific genes in equine

Identification of human chronic myelogenous leukemia progenitor cells with hemangioblastic characteristics.

Fang B, et al.

Blood, 105, 2733-2740 (2005)

Dose-dependent exacerbation of ventilation-induced lung injury by erythropoietin in preterm newborn lambs.

Beth J Allison et al.

Journal of applied physiology (Bethesda, Md. : 1985), 126(1), 44-50 (2018-11-02)

Erythropoietin (EPO) is being trialled in preterm infants to reduce brain injury, but high doses increase lung injury in ventilated preterm lambs. We aimed to determine whether early administration of lower doses of EPO could reduce ventilation-induced lung injury and

Erythropoietin induces bone marrow and plasma fibroblast growth factor 23 during acute kidney injury.

Luis Toro et al.

Kidney international, 93(5), 1131-1141 (2018-02-06)

It is accepted that osteoblasts/osteocytes are the major source for circulating fibroblast growth factor 23 (FGF23). However, erythropoietic cells of bone marrow also express FGF23. The modulation of FGF23 expression in bone marrow and potential contribution to circulating FGF23 has

PlanHab: Hypoxia counteracts the erythropoietin suppression, but seems to exaggerate the plasma volume reduction induced by 3 weeks of bed rest.

Keramidas ME, et al.

Physiological Reports, 4, e12760-e12760 (2016)

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