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SRP6217

Sigma-Aldrich

Fetuin A/ASHG human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

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Synonym(s):
Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

45-55 kDa

packaging

pkg of 10 and 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FETUA(197)

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SRP3283SAB4200820ECM001
Sigma-Aldrich

Sigma-Aldrich

SRP6217

Fetuin A/ASHG human

Sigma-Aldrich

Sigma-Aldrich

SRP3283

FETUIN A human

Fibronectin human recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Sigma-Aldrich

ECM001

Fibronectin human

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

-

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

-

assay

≥95% (SDS-PAGE)

form

lyophilized powder

form

lyophilized

form

-

form

lyophilized powder

mol wt

45-55 kDa

mol wt

45.0-55.0 kDa

mol wt

-

mol wt

259.5 kDa (The protein migrates as a higher band on SDS-PAGE due to glycosylation.)

packaging

pkg of 10 and 50 μg

packaging

pkg of 50 μg

packaging

antibody small pack of 25 μL

packaging

pkg of 200 μg

General description

Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β and BMP signaling proteins. Recombinant human Fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant Fetuin-A migrates at an apparent molecular weight of 45 – 55 kDa by SDS-PAGE run under non-reducing conditions.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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