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SRP6233

Sigma-Aldrich

Relaxin-3 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

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Synonym(s):
H3 relaxin, INSL7, Insulin-like peptide-7
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

mol wt

5.5 kDa

packaging

pkg of 10 μg
pkg of 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

Q8WXF3

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... RLN3(117579)

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INSL5/INSL7 HYBRID human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

assay

>80% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

form

lyophilized

mol wt

5.5 kDa

mol wt

predicted mol wt 23 kDa

mol wt

(Contains two disulfide-linked subunits: A chain ~2.8 kDa B chain ~3.3 kDa)

mol wt

5.7 kDa

packaging

pkg of 10 μg, pkg of 50 μg

packaging

-

packaging

-

packaging

pkg of 25 μg

General description

RLN3 (relaxin-3) is a relaxin peptide, belonging to the insulin superfamily, with each member containing two peptide subunits linked by three disulfide bridges. This protein shows a restricted expression pattern in the brain, mainly in lateral periaqueductal gray, nucleus incertus, and central gray regions in the brainstem. This protein is highly conserved across a range of species from fish to mammals.

Biochem/physiol Actions

RLN3 (relaxin-3) functions as a ligand for the leucine-rich repeat-containing G-protein-coupled receptor (LGR), LGR7. It also acts as a ligand for orphan receptor GPCR135, or SALPR (somatostatin- and angiotensin-like peptide receptor). RLN3 acts on LGR7 resulting in stimulation of adenylate cycle; RLN3 acts on GPCR135 to prevent activation of adenylate cyclase. The RLN3–GPCR135 signaling pathway, functions alongside GABA neurotransmitter in specific brain areas, and has regulatory effects on putative GABA neuronal networks. It also interacts with pathways containing CRF (corticotrophin releasing hormone)-like peptides and their receptors. CRF-like peptides and their receptors are implicated in stress-induced autonomic and behavioral changes. RLN3–GPCR135 system is overall involved in arousal, learning and memory, stress, and locomotor activity.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Relaxin-3 in GABA projection neurons of nucleus incertus suggests widespread influence on forebrain circuits via G-protein-coupled receptor-135 in the rat.
Ma S, et al.
Neuroscience, 144(1), 165-190 (2007)
Identification of relaxin-3/INSL7 as a ligand for GPCR142.
Liu C, et al.
The Journal of Biological Chemistry, 278(50), 50765-50770 (2003)
Central relaxin-3 administration causes hyperphagia in male Wistar rats.
McGowan BM, et al.
Endocrinology, 146(8), 3295-3300 (2005)

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