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SRP6233

Sigma-Aldrich

Relaxin-3 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

Synonym(s):

H3 relaxin, INSL7, Insulin-like peptide-7

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

lyophilized powder

mol wt

5.5 kDa

packaging

pkg of 10 μg
pkg of 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... RLN3(117579)

General description

RLN3 (relaxin-3) is a relaxin peptide, belonging to the insulin superfamily, with each member containing two peptide subunits linked by three disulfide bridges. This protein shows a restricted expression pattern in the brain, mainly in lateral periaqueductal gray, nucleus incertus, and central gray regions in the brainstem. This protein is highly conserved across a range of species from fish to mammals.

Biochem/physiol Actions

RLN3 (relaxin-3) functions as a ligand for the leucine-rich repeat-containing G-protein-coupled receptor (LGR), LGR7. It also acts as a ligand for orphan receptor GPCR135, or SALPR (somatostatin- and angiotensin-like peptide receptor). RLN3 acts on LGR7 resulting in stimulation of adenylate cycle; RLN3 acts on GPCR135 to prevent activation of adenylate cyclase. The RLN3–GPCR135 signaling pathway, functions alongside GABA neurotransmitter in specific brain areas, and has regulatory effects on putative GABA neuronal networks. It also interacts with pathways containing CRF (corticotrophin releasing hormone)-like peptides and their receptors. CRF-like peptides and their receptors are implicated in stress-induced autonomic and behavioral changes. RLN3–GPCR135 system is overall involved in arousal, learning and memory, stress, and locomotor activity.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Identification of relaxin-3/INSL7 as a ligand for GPCR142.
Liu C, et al.
The Journal of Biological Chemistry, 278(50), 50765-50770 (2003)
Relaxin-3 in GABA projection neurons of nucleus incertus suggests widespread influence on forebrain circuits via G-protein-coupled receptor-135 in the rat.
Ma S, et al.
Neuroscience, 144(1), 165-190 (2007)
Central relaxin-3 administration causes hyperphagia in male Wistar rats.
McGowan BM, et al.
Endocrinology, 146(8), 3295-3300 (2005)

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