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SRP6396

Fc-gamma RIIB/CD32b human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

CD32, CDw32, FCG2, FCGR2B, IGFR2

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

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biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 20.2 kDa
observed mol wt 23-33 kDa (DTT-reduced. Protein migrates due to different glycosylation. Ala 46 is the predicted N-terminus.)

packaging

pkg of 10 and 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P31994

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FCGR2B(2213)

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This Item
WH0002212M6SAB4500869SAB4301244
Fc-gamma RIIB/CD32b human recombinant, expressed in HEK 293 cells, &#8805;95% (SDS-PAGE)

Sigma-Aldrich

SRP6396

Fc-gamma RIIB/CD32b human

Monoclonal Anti-FCGR2A antibody produced in mouse clone 3E8, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

WH0002212M6

Monoclonal Anti-FCGR2A antibody produced in mouse

Anti-CD32 antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4500869

Anti-CD32 antibody produced in rabbit

Anti-phospho-CD32 (pTyr292) antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4301244

Anti-phospho-CD32 (pTyr292) antibody produced in rabbit

assay

≥95% (SDS-PAGE)

assay

-

assay

-

assay

-

recombinant

expressed in HEK 293 cells

recombinant

-

recombinant

-

recombinant

-

biological source

human

biological source

mouse

biological source

rabbit

biological source

rabbit

mol wt

calculated mol wt 20.2 kDa, observed mol wt 23-33 kDa (DTT-reduced. Protein migrates due to different glycosylation. Ala 46 is the predicted N-terminus.)

mol wt

-

mol wt

antigen 34 kDa

mol wt

32 kDa

form

lyophilized

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

impurities

<1 EU/μg endotoxin (LAL test)

impurities

-

impurities

-

impurities

-

General description

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells down regulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
6G24L73880
3E05L73880
3G18L73880

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Esteban Cruz et al.
Pharmaceutics, 13(11) (2021-11-28)
Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives. Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity

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