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  • SRP6396
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SRP6396

Sigma-Aldrich

Fc-gamma RIIB/CD32b human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

All Photos(1)
Synonym(s):
CD32, CDw32, FCG2, FCGR2B, IGFR2
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 20.2 kDa
observed mol wt 23-33 kDa (DTT-reduced. Protein migrates due to different glycosylation. Ala 46 is the predicted N-terminus.)

packaging

pkg of 10 and 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P31994

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FCGR2B(2213)

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Fc-gamma RIIB/CD32b human recombinant, expressed in HEK 293 cells, &#8805;95% (SDS-PAGE)

Sigma-Aldrich

SRP6396

Fc-gamma RIIB/CD32b human

Sigma-Aldrich

Sigma-Aldrich

SRP0604

Human CD226, FC Fusion

CD319/SLAMF7/CRACC human recombinant, expressed in HEK 293 cells, &#8805;95% (SDS-PAGE)

Sigma-Aldrich

SRP6383

CD319/SLAMF7/CRACC human

Fc gamma RIIIB/CD16b human recombinant, expressed in HEK 293 cells, &#8805;98% (SDS-PAGE)

Sigma-Aldrich

SRP6451

Fc gamma RIIIB/CD16b human

biological source

human

biological source

-

biological source

human

biological source

human

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

assay

-

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

form

lyophilized

form

frozen liquid

form

lyophilized

form

lyophilized

mol wt

calculated mol wt 20.2 kDa, observed mol wt 23-33 kDa (DTT-reduced. Protein migrates due to different glycosylation. Ala 46 is the predicted N-terminus.)

mol wt

52.8 kDa

mol wt

calculated mol wt 23.2 kDa, observed mol wt 33-48 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 23 is the predicted N-terminus.)

mol wt

calculated mol wt 21.7 kDa, observed mol wt 38-50 kDa (DTT-reduced. Protein migrates due to glycosylation. Gly 17 is the predicted N-terminal.)

packaging

pkg of 10 and 50 μg

packaging

-

packaging

pkg of 10 μg, pkg of 50 μg

packaging

pkg of 10 and 50 μg

General description

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells down regulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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