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SRP6415

Sigma-Aldrich

Cathepsin D human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

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Synonym(s):
CLN10, CPSD, CTSD
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 43.6 kDa
observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CTSD(1509)

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This Item
C0715SAB4200767SRP6414
Cathepsin D human recombinant, expressed in HEK 293 cells, &#8805;95% (SDS-PAGE)

Sigma-Aldrich

SRP6415

Cathepsin D human

Cathepsin B human recombinant, expressed in HEK 293 cells, &#8805;95% (SDS-PAGE)

Sigma-Aldrich

SRP6414

Cathepsin B human

recombinant

expressed in HEK 293 cells

recombinant

-

recombinant

-

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

assay

-

assay

-

assay

≥95% (SDS-PAGE)

form

lyophilized

form

-

form

buffered aqueous solution

form

lyophilized

mol wt

calculated mol wt 43.6 kDa, observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)

mol wt

antigen 34 kDa, antigen 52 kDa (weaker band)

mol wt

-

mol wt

calculated mol wt 29 kDa (Mature), calculated mol wt 36.7 kDa (Pro), observed mol wt 34 kDa, observed mol wt 43_kDaand34 kDa (DTT-reduced. Protein migrates due to glycosylation. Arg18 (pro) or Phe74 (mature) is the predicted N-terminal.)

impurities

<1 EU/μg endotoxin (LAL test)

impurities

-

impurities

-

impurities

<1 EU/μg endotoxin (LAL test)

General description

Cathepsin D is also known as CTSD, CPSD, which belongs to the peptidase A1 family. Cathepsin D can be cleaved into the following 2 chains: cathepsin D light chain and cathepsin D heavy chain, which is expressed in the aorta extracellular space (at protein level). The catalytic activity of Cathepsin D is specificity similar to, but narrower than, that of pepsin A. Cathepsin D does not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Cathepsin D involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
This gene is mapped to human chromosome 11p15.5.

Biochem/physiol Actions

Cathepsin D (CatD) takes part in the intracellular degradation of advanced glycation end (AGE) products. AGE deposition is predominant in photoaged skin. Thus, CatD might be useful in antiphotoaging therapy. Elevated circulating CatD is observed in type 2 diabetes and can be considered as an important biomarker for type 2 diabetes dependent cardiac dysfunction. Increased CatD, is observed in non-alcoholic steatohepatitis.

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Cluster analysis of risk factor genetic polymorphisms in Alzheimer?s disease
Randall CN, et al.
Neurochemical Research, 34(1), 23-28 (2009)
Increased Cathepsin D Correlates with Clinical Parameters in Newly Diagnosed Type 2 Diabetes
Liu L, et al.
Disease Markers, 2017(1), 23-28 (2017)
Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention
Walenbergh SMA, et al.
Scientific reports, 6(1), 38278-38278 (2016)
Xiaolai Zhou et al.
Molecular neurodegeneration, 12(1), 62-62 (2017-08-25)
Mutations resulting in progranulin (PGRN) haploinsufficiency cause frontotemporal lobar degeneration with TDP-43-positive inclusions (FTLD-TDP), a devastating neurodegenerative disease. PGRN is localized to the lysosome and important for proper lysosome function. However, the metabolism of PGRN in the lysosome is still
Clarissa Valdez et al.
Human molecular genetics, 26(24), 4861-4872 (2017-10-17)
Frontotemporal dementia (FTD) encompasses a group of neurodegenerative disorders characterized by cognitive and behavioral impairments. Heterozygous mutations in progranulin (PGRN) cause familial FTD and result in decreased PGRN expression, while homozygous mutations result in complete loss of PGRN expression and

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