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SRP6455

Sigma-Aldrich

EpCAM/CD326 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

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Synonym(s):
DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK1, TACSTD1, TROP1
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 29 kDa (after removal of signal peptide.)
observed mol wt 33-36 kDa by SDS-PAGE (DTT-reduced. rh EpCAMProtein migrates as poly peptide due to glycosylation. Gln24 is the predicted N-terminal.)

packaging

pkg of 10 and 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EPCAM(4072)

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MABT1587SAB4701095SAB4700848
vibrant-m

SRP6455

EpCAM/CD326 human

recombinant

expressed in HEK 293 cells

recombinant

-

recombinant

-

recombinant

-

biological source

human

biological source

mouse

biological source

mouse

biological source

mouse

form

lyophilized

form

-

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

calculated mol wt 29 kDa (after removal of signal peptide.), observed mol wt 33-36 kDa by SDS-PAGE (DTT-reduced. rh EpCAMProtein migrates as poly peptide due to glycosylation. Gln24 is the predicted N-terminal.)

mol wt

calculated mol wt 34.93 kDa, observed mol wt ~36 kDa

mol wt

-

mol wt

-

impurities

<1 EU/μg endotoxin (LAL test)

impurities

-

impurities

-

impurities

-

General description

Epithelial cell adhesion molecule (EpCAM) is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A (mAb) antigen. The gene encoding EpCAM is localized on human chromosome 2p21.

Biochem/physiol Actions

Epithelial cell adhesion molecule (EpCAM) is intricately linked with the cadherin-catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The protein has several cellular functions, like epithelial-specific cell-adhesion, cell signaling, migration, proliferation and differentiation. It is expressed in most epithelial malignancies (like epithelial ovarian cancer) and was reported as a tumor marker or a candidate of molecular targeting therapy. EpCAM cross signaling with N-cadherin involves phosphoinositide-3-kinase (Pi3K), resulting in the abrogation of the cadherin adhesion complexes in epithelial cells.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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The Epithelial Cell Adhesion Molecule (Ep-CAM) as a Morphoregulatory Molecule Is a Tool in Surgical Pathology
Manon J. Winter
The American Journal of Pathology, 163(6), 2139-2148 (2003)
Epithelial cell adhesion molecule expression (CD326) in cancer: a short review.
Patriarca C
Cancer Treatment Reviews, 38(1), 68-75 (2012)
Overexpression of epithelial cell adhesion molecule (Ep-CAM) is an independent prognostic marker for reduced survival of patients with epithelial ovarian cancer.
Spizzo G
Gynecologic Oncology, 103(2), 483-488 (2006)
Cadherins are regulated by Ep-CAM via phosphaditylinositol-3 kinase.
Winter MJ
Molecular and Cellular Biochemistry, 302(1-2), 19-26 (2007)
The evolving roles of canonical WNT signaling in stem cells and tumorigenesis: implications in targeted cancer therapies
Ke Yang
Laboratory Investigation; a Journal of Technical Methods and Pathology, 96, 116-136 (2016)

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