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SRP8053

Sigma-Aldrich

IL-35 (mouse): FC (human)

recombinant, expressed in CHO cells

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Synonym(s):
Interleukin-35, T-cell immunoglobulin and mucin domain-containing protein 4
NACRES:
NA.32

biological source

human
mouse

recombinant

expressed in CHO cells

assay

≥98% (SDS-PAGE)

form

lyophilized

mol wt

monomer 23 kDa by calculation (IL-12a (p35))
monomer 46 kDa by calculation (Ebi3)

packaging

pkg of 5 μg

impurities

<0.06 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

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recombinant

expressed in CHO cells

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in CHO cells

biological source

human, mouse

biological source

human

biological source

human

biological source

mouse

form

lyophilized

form

(Lyophilized with no additives from a 0.2µ filtered solution)

form

lyophilized

form

lyophilized

packaging

pkg of 5 μg

packaging

pkg of 50 μg

packaging

pkg of 10 μg

packaging

pkg of 100 μg

UniProt accession no.

O35228, P43431

UniProt accession no.

P60568

UniProt accession no.

P60568

UniProt accession no.

Q8VIM0

General description

Interleukin-35 (IL-35) is a novel IL-12 family cytokine produced by regulatory T cells (Treg) but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of EBI3 (Epstein-Barr-Virus-induced gene 3) and IL-12a (p35). EBI3 is a downstream target of Foxp3, a transcription factor required for Treg-cell development and function, and thus Treg-cell restriction of IL35 occurs.

Application

IL-35 has been used to study its immunosuppressive effect on acute graft-versus-host disease in a mouse model.

Biochem/physiol Actions

Regulatory T cells are essential for maintaining self tolerance and preventing autoimmunity, and IL-35 is identified as a molecule that mediates the immune suppression function of Treg-cell. As an inhibitory cytokine, IL-35 induces proliferation of Treg-cell populations but suppresses Th17 cell development. Studies in mice show the absence of either IL-35 chain from Treg-cell reduces the cells′ ability to suppress inflammation using an experimental model for inflammatory bowel disease. IL-35 is suggested as a potential target of immunotherapy.

Physical form

Lyophilized from 0.2 μm-filtered solution in PBS.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Other Notes

The mouse IL-35 complex composed of the Ebi3 subunit (aa 23-228) and the IL-12a (p35) subunit (aa 23-215) is fused through a polypeptide linker to the Fc region of human IgG1.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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IL-35 inhibits acute graft-versus-host disease in a mouse model
Zhang X H, et al.
International Immunopharmacology, 29(2), 383-392 (2015)
The inhibitory cytokine IL-35 contributes to regulatory T-cell function.
Collison LW
Nature, 450, 566-569 (2007)
Chen Xu et al.
Neurochemical research, 43(7), 1454-1463 (2018-06-20)
IL-35 has been identified as a novel anti-inflammatory cytokine that belongs to the IL-12 cytokine family and has been verified to play a protective role in autoimmune diseases. In this study, we investigated the protective effects of IL-35 on cerebral
IL-35 is a novel cytokine with therapeutic effects against collagen-induced arthritis through the expansion of regulatory T cells and suppression of Th17 cells.
Niedbala W
European Journal of Immunology, 37, 3021-3029 (2007)
Donghong Chen et al.
Respiratory research, 22(1), 280-280 (2021-10-30)
IL-35 subunit EBI3 is up-regulated in pulmonary fibrosis tissues. In this study, we investigated the pathological role of EBI3 in pulmonary fibrosis and dissected the underlying molecular mechanism. Bleomycin-induced pulmonary fibrosis mouse model was established, and samples were performed gene

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