STRN10

Sigma-Aldrich

Spectrum Plant Total RNA Kit

greener alternative

sufficient for 10 purifications

NACRES:
NA.52

usage

sufficient for 10 purifications

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

storage temp.

15-25°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Legal Information

Spectrum is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

RIDADR

UN 3316 9

Flash Point(F)

154.4 °F

Flash Point(C)

68 °C

Certificate of Analysis
Certificate of Origin
Qun Cheng et al.
Frontiers in plant science, 11, 272-272 (2020-04-08)
Soybean [Glycine max (L.) Merr.] is an important crop for oil and protein resources worldwide, and its farming is impacted by increasing soil salinity levels. In Arabidopsis the gene EARLY FLOWERING 3 (ELF3), increased salt tolerance by suppressing salt stress...
Genomic approaches for interrogating the biochemistry of medicinal plant species.
Gongora-Castillo E
Methods in Enzymology, 517, 139-159 (2012)
De novo Assembly of Leaf Transcriptome in the Medicinal Plant Andrographis paniculata.
Cherukupalli N
Frontiers in Plant Science, 7, 1203-1203 (2016)
Doug J Hinchliffe et al.
BMC genomics, 12, 445-445 (2011-09-10)
Cotton fiber length is an important quality attribute to the textile industry and longer fibers can be more efficiently spun into yarns to produce superior fabrics. There is typically a negative correlation between yield and fiber quality traits such as...
Zhandong Cai et al.
Frontiers in plant science, 11, 724-724 (2020-06-26)
Cadmium (Cd) is a widespread pollutant that is toxic to living organisms. Previous studies have identified certain WRKY transcription factors, which confer Cd tolerance in different plant species. In the present study, we have identified 29 Cd-responsive WRKY genes in...
Related Content
The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.
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