STRN250

Sigma-Aldrich

Spectrum Plant Total RNA Kit

greener alternative

sufficient for 250 purifications

NACRES:
NA.52

Quality Level

usage

sufficient for 250 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

storage temp.

15-25°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.

Application

Spectrum®Plant Total RNA Kit has been used:
  • to extract RNA from the material ground in liquid nitrogen
  • to isolate total RNA from mutant and wt (wild type) plantlets
  • to extract total RNA from leaf samples
  • to extract RNA from all plant tissues

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Legal Information

Specac is a registered trademark of Specac Ltd.
Spectrum is a trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
Certificate of Origin
Zhonghua Li et al.
The New phytologist, 226(6), 1738-1752 (2020-02-06)
The cotton fibre serves as a valuable experimental system to study cell wall synthesis in plants, but our understanding of the genetic regulation of this process during fibre development remains limited. We performed a genome-wide association study (GWAS) and identified...
Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness
Minina EA, et al.
Journal of Experimental Botany, 69(6), 1415-1432 (2018)
Genome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens.
O'Donoghue MT
Journal of Experimental Botany, 64(12), 3567-3581 (2013)
Knockout mutants as a tool to identify the subunit composition of Arabidopsis glutamine synthetase isoforms
Dragicevic M, et al.
Plant Physiology and Biochemistry, 79(6), 1-9 (2014)
Transcriptomic Analysis of Drought-Sensitive and Tolerant Genotypes of Perennial Ryegrass (Lolium perenne L.)
Breeding Grasses and Protein Crops in the Era of Genomics, 79(6), 160-165 (2018)
Related Content
The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.
Read More
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Read More

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