STRN50

Sigma-Aldrich

Spectrum Plant Total RNA Kit

greener alternative

sufficient for 50 purifications

NACRES:
NA.52
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 50 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

storage temp.

20-25°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.

Application

Spectrum® Plant Total RNA Kit has been used to isolate RNA from ground fiber tissue of cultured cotton fiber, from neem tissues and from Sarracenia, an insectivorous plant.

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Legal Information

SabreLite is a registered trademark of Pelican Products, Inc.
Spectrum is a trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
Certificate of Origin
Soybean DICER-LIKE2 Regulates Seed Coat Color via Production of Primary 22-Nucleotide Small Interfering RNAs from Long Inverted Repeats.
Jia, et al.
Plant Cell, 32, 3662-3673 (2021)
Meng Zhang et al.
Journal of experimental botany, 71(3), 951-969 (2019-10-23)
Anther development in flowering plants is highly sensitive to high-temperature (HT) stress. Understanding the potential epigenetic mechanism of anther infertility induced by HT stress in cotton (Gossypium hirsutum L.) is crucial for the effective use of genetic resources to guide...
A synthetic auxin (NAA) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber.
Singh B
Plant Cell Reports, 28(7), 1023-1032 (2009)
Nikolaj L Hansen et al.
Microbial cell factories, 19(1), 15-15 (2020-01-30)
Celastrol is a promising anti-obesity agent that acts as a sensitizer of the protein hormone leptin. Despite its potent activity, a sustainable source of celastrol and celastrol derivatives for further pharmacological studies is lacking. To elucidate the celastrol biosynthetic pathway...
Prasanna Kankanala et al.
Plant, cell & environment, 43(4), 1084-1101 (2020-01-14)
Necrotrophic fungi constitute the largest group of plant fungal pathogens that cause heavy crop losses worldwide. Phymatotrichopsis omnivora is a broad host, soil-borne necrotrophic fungal pathogen that infects over 2,000 dicotyledonous plants. The molecular basis of such broad host range...
Articles
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
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Related Content
The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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