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T0440

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA

peroxidase substrate

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Synonym(s):
TMB
NACRES:
NA.83

Quality Level

form

liquid

storage temp.

2-8°C

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This Item
T8665T4444T4319
storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

300

Quality Level

-

Quality Level

-

General description

TMB substrate is a chromogenic substrate for horseradish peroxidase - HRP.

Application

TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm.

How to use our TMB substrate:
  • Our TMB substrate is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase.
  • Upon reaching your assay endpoint with addition of acid, measure the TMB substrate intensity at 450 nm.
  • Our TMB Substrate is recommended for ELISA (microwell) procedures, and has been used in various assays, as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein, tetracosactide, sIgA and neutrophil extracellular traps (NETs).
  • 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System is not recommended for membrane applications. Since TMB substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
Note:TMB substrate is light-sensitive and should be protected from direct sunlight or UV sources.

recommended

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Gan EH, et al.
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Lim PW, et al.
BioMed Research International, 2019 (2019)
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Clinica chimica acta; international journal of clinical chemistry, 473, 127-132 (2017-08-29)
Heat shock proteins (HSPs) represent intracellular mechanisms of stress response. Clinical implications of their (systemic) expression in patients with chronic heart failure (HF) remain inconclusive. In outpatients with chronic stable HF plasma HSP27 levels were measured using ELISA. Patients were
Marco Maccarini et al.
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OprF has a central role in Pseudomonas aeruginosa virulence and thus provides a putative target for either vaccines or antibiotic cofactors that could overcome the bacterium's natural resistance to antibiotics. Here we describe a procedure to optimize the production of
R Roth et al.
Journal of applied microbiology, 122(5), 1402-1411 (2017-03-10)
To investigate the production of soluble cross-reacting material 197 (CRM The use of co-expression of a sulphydryl oxidase (SOX) and protein disulphide isomerase for the production of soluble CRM SOX and protein disulphide isomerase are enzymes involved in the formation

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