T0665

holo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥97%

• Synonym(s): Siderophilin, iron-saturated
• CAS Number: 11096-37-0
• EC Number: 234-318-8
• MDL number: MFCD00131337
• NACRES: NA.75

Properties

• biological source: human plasma
• Quality Level: 200
• description: Iron-saturated
• product line: BioReagent
• Assay: ≥97%
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: HIV and HBsAg, none detected; endotoxin, tested
• solubility: H₂O: 50 mg/mL
• UniProt accession no.: P02787
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... TF(7018)

Description

Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.

Biochem/physiol Actions

Provides iron to cells in a physiologically stable and safe form.

Preparation Note

Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Analysis Note

Iron content by ICP reported on a lot-specific basis.

Purity by agarose gel electrophoresis.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3