T0825

Sigma-Aldrich

Monoclonal Anti-Transportin 1 antibody produced in mouse

clone D45, purified immunoglobulin, buffered aqueous solution

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

D45, monoclonal

form

buffered aqueous solution

mol wt

antigen ~90 kDa

species reactivity

human, mouse, rat, canine, bovine

application(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using HeLa nuclear extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TNPO1(3842)
mouse ... Tnpo1(238799)
rat ... Tnpo1(309126)

General description

Transportin 1 (TNPO1 or TRN1) also known as karyopherin-β2, is a nuclear import protein and belongs to importin-β family. In the human chromosome the TNPO1 gene is localized on 5q13.2.(10)

Immunogen

recombinant human transportin 1.

Application

Monoclonal Anti-Transportin 1 antibody produced in mouse has been used in western blotting, immunofluorescence, and immunostaining.

Packaging

200 μL in glass bottle

Biochem/physiol Actions

Transportin 1 (TNPO1 or TRN1) recognizes the nuclear localization signals (NLS) and binds to the nuclear pore complex (NPC) to facilitate the transport of substances from cytoplasm to nucleus. TRN1 transports viral, and ribosomal proteins with the aid of RanGTP (Ras associated nuclear protein-GTP). TRN1 is also known to regulate the key events in the cell cycle assembly like nuclear membrane and nuclear pore. Mutations in TRN1 might cause amyotrophic lateral sclerosis due to mislocalisation of FUS (fused in sarcoma) protein. TRN1 is associated with regulation of circadian rhythm through nuclear localization of PER1 (period circadian protein homolog 1) protein.

Target description

Transportin 1 encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Structural basis for substrate recognition and dissociation by human transportin 1
Imasaki T, et al.
Molecular Cell, 28(1), 57-67 (2007)
Transcriptional regulation of importin-alpha1 by JunD modulates subcellular localization of RNA-binding protein HuR in intestinal epithelial cells
Xu Y, et al.
American Journal of Physiology. Cell Physiology, 311(6), C874-C883 (2016)
Saskia Hutten et al.
Cell reports, 33(12), 108538-108538 (2020-12-29)
Nuclear import receptors, also called importins, mediate nuclear import of proteins and chaperone aggregation-prone cargoes (e.g., neurodegeneration-linked RNA-binding proteins [RBPs]) in the cytoplasm. Importins were identified as modulators of cellular toxicity elicited by arginine-rich dipeptide repeat proteins (DPRs), an aberrant...
IPO3 mediated nonclassical nuclear import of NEMO drives DNA damage-dependent NF-kappaB activation
Hwang B, et al.
The Journal of Biological Chemistry, jbc-M115 (2015)
microRNA miR-128 represses LINE-1 retrotransposition by downregulating the nuclear import factor TNPO1
Idica A, et al.
The Journal of Biological Chemistry, 28(1), jbc-M117 (2017)

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