T0825

Sigma-Aldrich

Monoclonal Anti-Transportin 1 antibody produced in mouse

clone D45, purified immunoglobulin, buffered aqueous solution

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

D45, monoclonal

form

buffered aqueous solution

mol wt

antigen ~90 kDa

species reactivity

human, mouse, rat, canine, bovine

application(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using HeLa nuclear extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TNPO1(3842)
mouse ... Tnpo1(238799)
rat ... Tnpo1(309126)

General description

Transportin 1 (TNPO1 or TRN1) also known as karyopherin-β2, is a nuclear import protein and belongs to importin-β family. In the human chromosome the TNPO1 gene is localized on 5q13.2.(10)

Immunogen

recombinant human transportin 1.

Application

Monoclonal Anti-Transportin 1 antibody produced in mouse has been used in western blotting, immunofluorescence, and immunostaining.

Packaging

200 μL in glass bottle

Biochem/physiol Actions

Transportin 1 (TNPO1 or TRN1) recognizes the nuclear localization signals (NLS) and binds to the nuclear pore complex (NPC) to facilitate the transport of substances from cytoplasm to nucleus. TRN1 transports viral, and ribosomal proteins with the aid of RanGTP (Ras associated nuclear protein-GTP). TRN1 is also known to regulate the key events in the cell cycle assembly like nuclear membrane and nuclear pore. Mutations in TRN1 might cause amyotrophic lateral sclerosis due to mislocalisation of FUS (fused in sarcoma) protein. TRN1 is associated with regulation of circadian rhythm through nuclear localization of PER1 (period circadian protein homolog 1) protein.

Target description

Transportin 1 encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Nuclear pore complex protein mediated nuclear localization of dicer protein in human cells
Ando Y, et al.
PLoS ONE, 6(8), e23385-e23385 (2011)
Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function
Hock EM, et al.
Cell Reports, 24(4), 987-1000 (2018)
Benjamin Bourgeois et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(15), 8503-8514 (2020-04-03)
The specific interaction of importins with nuclear localization signals (NLSs) of cargo proteins not only mediates nuclear import but also, prevents their aberrant phase separation and stress granule recruitment in the cytoplasm. The importin Transportin-1 (TNPO1) plays a key role...
The non-classical nuclear import carrier Transportin 1 modulates circadian rhythms through its effect on PER1 nuclear localization
Korge S, et al.
PLoS Genetics, 14(1), e1007189-e1007189 (2018)
Structural basis for substrate recognition and dissociation by human transportin 1
Imasaki T, et al.
Molecular Cell, 28(1), 57-67 (2007)

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