Tissue plasminogen activator (tPA) is a protease of the S1 family (trypsin family) found in various mammalian tissues, especially endothelial cells. tPA is secreted as a single-chain precursor, which is cleaved to a two-chain form by plasmin. It is supplied in the single-chain form with a molecular weight of approximately 70 kDa. The Tissue Plasminogen Activator (tPA) gene is mapped to human chromosome 8p11.21 and encodes a protease of 69 kDa, primarily containing serine amino acid residue. Tissue plasminogen activator (tPA) is a protein primarily composed of the elements found in amino acids: carbon (C), hydrogen (H), oxygen (O), nitrogen (N), and sulfur (S).
T0831
Tissue plasminogen activator human
Synonym(s):
tPA
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100 μG
$595.00
1 MG
$1,340.00
About This Item
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form
lyophilized powder
Quality Level
specific activity
≥300 KU/mg protein
mol wt
~70 kDa
impurities
HIV, HCV, and HBsAg, tested negative
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... PLAT(5327)
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This Item | SAB5701512 | 528178 | 528175 |
|---|---|---|---|
| Gene Information human ... PLAT(5327) | Gene Information human ... PLAT(5327) | Gene Information - | Gene Information - |
| specific activity ≥300 KU/mg protein | specific activity - | specific activity 7 units/mg protein | specific activity ≥120 units/mg protein |
| form lyophilized powder | form liquid | form lyophilized | form lyophilized |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~70 kDa | mol wt - | mol wt - | mol wt - |
General description
Tissue plasminogen activator (tPA) is a protease of the S1 family (trypsin family) and is found in a wide variety of mammalian tissues, especially endothelial cells. tPA is secreted as a single chain precursor, which is cleaved to a two-chain form by plasmin.[1] Supplied in the single chain form with a molecular weight of ~70 kDa.[2] The Tissue Plasminogen Activator (tPA) gene is mapped to human chromosome 8p11.21 and encodes a protease of 69 kDa majorly containing serine amino acid residue. It is predominantly expressed in endothelial cells.[3][4][5]
Application
Tissue plasminogen activator has been used in a study to assess treatment risks of intracranial hemorrhage among patients with acute ischemic stroke.[6] Tissue plasminogen activator has also been used in a study to investigate the successful treatment of early thrombosis of HeartWare left ventricular assist devices with intraventricular thrombolytics.[7] Human tissue plasminogen activator has been used to study the effect of tyrosinase-related protein 2 (TRP-2) expression on melanin production in sheep.[8]
Biochem/physiol Actions
tPA is a potent activator of fibrinolysis and converts plasminogen into plasmin and also initiates cell signaling.[9][3] The plasminogen activation system may also be associated with degradation of amyloid fibrils.[10] It is also known to mediate inflammatory and extracellular matrix remodeling processes and is predicted to participate in disease progression of intracranial aneurysm indicating brain vascular malformations. Upregulation of the gene is observed in aortic aneurysms.[3]
Physical form
Lyophilized powder, from 100 mM PBS (pH 7.4), 3.5 mg/mL L-arginine, and 0.001% Tween® 80
Preparation Note
Reconstitute with dH2O to a final concentration of 1 mg/mL (e.g. 100 μg should be reconstituted in 100 μL).
Legal Information
TWEEN is a registered trademark of Croda International PLC
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Tissue-type plasminogen activator controls neuronal death by raising surface dynamics of extrasynaptic NMDA receptors.
Lesept F, et al.
Cell Death & Disease, 7(11), e2466-e2466 (2016)
Intra-arterial prourokinase for acute ischemic stroke: the PROACT II study: a randomized controlled trial.
Furlan A, et al.
JAMA : The Journal of the American Medical Association, 282(21), 2003-2011 (1999)
Tissue-type plasminogen activator regulates macrophage activation and innate immunity.
Mantuano E, et al.
Blood (2017)
Successful treatment of early thrombosis of HeartWare left ventricular assist device with intraventricular thrombolytics.
Kamouh A, et al.
The Annals of Thoracic Surgery, 94(1), 281-283 (2012)
Converting Tissue Type Plasminogen Activator into a Zymogen IMPORTANT ROLE OF Lys156.
Tachias K and Madison E L
The Journal of Biological Chemistry, 272(1), 28-31 (1997)
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What is the elemental composition of the tissue plasminogen activator?
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What are the storage conditions and length for the reconstituted product?
1 answer-
Following reconstitution, aliquot and freeze at -20°C to -70°C. Stock solutions are stable for up to 6 months. Avoid repeated freeze/thaw cycles. For additional stability a protease-free HSA or BSA may be added at 1% to the stock solution.
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