This product is suitable for cell culture. Aqueous solutions of apo-transferrin should be stable at 4°C for 5–10 days. Solutions should be filter-sterilized for maximum stability. For more information, please refer to the product information sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/136/004/t1147pis.pdf
T1147
apo-Transferrin human
powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
apo-Transferrin human
Synonym(s):
Human transferrin, Siderophilin
Sign In to View Organizational & Contract Pricing.
Select a Size
100 MG
$243.00
500 MG
$755.00
5 X 100 MG
$891.00
1 G
$1,240.00
5 G
Inquire
About This Item
Skip To
Product Name
apo-Transferrin human, powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
biological source
human plasma
product line
BioReagent
assay
≥98% (agarose gel electrophoresis)
form
powder
mol wt
76-81 kDa
concentration
~25 mM
technique(s)
cell culture | mammalian: suitable
impurities
HIV and HBsAg, source material tested negative
≤0.005% iron
solubility
H2O: 50 mg/mL
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... TF(7018)
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | T1428 | T0665 | T3705 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) immunoprecipitation (IP): suitable, tissue culture: suitable |
| form powder | form powder | form powder | form powder |
| biological source human plasma | biological source bovine | biological source human plasma | biological source human |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in - |
| solubility H2O: 50 mg/mL | solubility H2O: 10 mg/mL | solubility H2O: 50 mg/mL | solubility - |
Application
Apo-Transferrin human can be supplemented with iron or used to bind free iron present in media. It is also used to transport iron into mammalian cells.
Biochem/physiol Actions
Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human apo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Apo-transferrin can be loaded with iron prior to use or added directly to an iron containing medium.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Other Notes
Non-heme iron-transport protein.
Storage Class
11 - Combustible Solids
wgk
WGK 3
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Giang D Nguyen et al.
PloS one, 9(5), e96858-e96858 (2014-05-08)
H1 linker histone proteins are essential for the structural and functional integrity of chromatin and for the fidelity of additional epigenetic modifications. Deletion of H1c, H1d and H1e in mice leads to embryonic lethality by mid-gestation with a broad spectrum
Giang D Nguyen et al.
PloS one, 8(8), e72698-e72698 (2013-08-24)
Huntington's disease (HD) is a neurodegenerative disease caused by abnormal polyglutamine expansion in the huntingtin protein (Htt). Although both Htt and the HD pathogenic mutation (mHtt) are implicated in early developmental events, their individual involvement has not been adequately explored.
Kamil Strzelak et al.
Analytica chimica acta, 995, 43-51 (2017-11-12)
The Multicommutated Flow Analysis (MCFA) system for the estimation of clinical iron parameters: Serum Iron (SI), Unsaturated Iron Binding Capacity (UIBC) and Total Iron Binding Capacity (TIBC) has been proposed. The developed MCFA system based on simple photometric detection of
Kathleen N O'Guin et al.
ASN neuro, 6(1), e00135-e00135 (2014-01-31)
The molecular requirements for human myelination are incompletely defined, and further study is needed to fully understand the cellular mechanisms involved during development and in demyelinating diseases. We have established a human co-culture model to study myelination. Our earlier observations
Agla J Fridriksdottir et al.
Nature communications, 6, 8786-8786 (2015-11-14)
Investigating the susceptibility of oestrogen receptor-positive (ER(pos)) normal human breast epithelial cells (HBECs) for clinical purposes or basic research awaits a proficient cell-based assay. Here we set out to identify markers for isolating ER(pos) cells and to expand what appear
Articles
Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.
Related Content
Instructions
-
Is this product sterile and can be used directly in cell culture media or does it have to be filtered first?
1 answer-
Helpful?
-
-
What is the carbohydrate content of human transferrin?
1 answer-
We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service