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T1165

Sigma-Aldrich

Tris-Tricine-SDS Buffer 10× Concentrate

Synonym(s):

10X TTS Buffer, 10X Tris Tricine SDS running buffer, Tris Tricine SDS transfer buffer

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500 ML
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500 ML
$138.57

About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

$138.57

List Price$149.00Save 7%
Web-Only Promotion

In StockDetails


Request a Bulk Order

sterility

0.2 μm filtered

Quality Level

form

liquid

pH

8.05-8.30 (25 °C in water, high purity, 1:10)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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This Item
T7777SRE0062T4904
Quality Level

200

Quality Level

200

Quality Level

500

Quality Level

200

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

form

liquid

form

liquid

form

liquid

form

-

pH

8.05-8.30 (25 °C in water, high purity, 1:10)

pH

-

pH

-

pH

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

sterility

0.2 μm filtered

General description

Tris-Tricine-SDS (TTS) running buffer is the cathode (upper reservoir) buffer for SDS−polyacrylamide gel electrophoresis of proteins using the Schagger and von Jagow method. The Schagger and von Jagow method is designed for the separation of small molecular weight proteins. It differs from the Laemmli method in that the glycine is replaced with tricine and the gel contains 1M Tris-HCl, pH 8.45 instead of 0.375 M Tris-HCl, pH 8.9. Dilution of the 10X TTS buffer produces a 1X cathode buffer containing 100 mM Tris, 100 mM tricine and 0.1% SDS. Recommended running conditions is 125 volts.

Application

Tris-Tricine-SDS Buffer 10× Concentrate is suitable for use as a running buffer for polyacrylamide based separation of bronchoalveolar lavage fluid (BALF).[1]

Other Notes

1M Tris, 1M Tricine, 1% (w/v) SDS, pH approx. 8.2.

Analysis Note

Tested for use as a cathode buffer for Tris-Tricine gel electrophoresis to separate low molecular weight proteins.

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