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T1378

Sigma-Aldrich

Sigma 7-9®

≥99% (titration), crystalline

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Synonym(s):
Trizma® base, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

description

aminopeptidase substrate

Quality Level

Assay

≥99% (titration)

form

crystalline

impurities

≤0.5% water (Karl Fischer)

color

white to off-white

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: 0.667 g/mL, clear, colorless to faintly yellow

absorption

≤0.4 at 290 at 40%

application(s)

diagnostic assay manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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1 of 4

This Item
T6791T4661T1503
Sigma 7-9® ≥99% (titration), crystalline

Sigma-Aldrich

T1378

Sigma 7-9®

-
Trizma® base BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

Sigma-Aldrich

T6791

Trizma® base

Premium Grade
Trizma® base ≥99.9% (titration), crystalline

Sigma-Aldrich

T4661

Trizma® base

-
Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline

Sigma-Aldrich

T1503

Trizma® base

-
form

crystalline

form

crystalline

form

crystalline

form

crystalline

impurities

≤0.5% water (Karl Fischer)

impurities

≤0.005% Insoluble matter

impurities

-

impurities

-

color

white to off-white

color

-

color

white

color

-

pH

10.5-12

pH

10.5-12.0 (1 M in H2O)

pH

10.0-11.5

pH

10.5-12

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

General description

Sigma 7-9® also known as Trizma base or tris(hydroxymethyl)aminomethane, is a versatile biological buffer used in various applications, such as protein freezing, electrophoresis, and the preparation of buffer solutions such as TAE and TBE. With a pKa of 8.06, Tris is particularly valuable in labs working within the typical physiological pH range of living organisms (pH 7.0-9.0). It is also commonly employed in combination with other buffering agents to precisely adjust solution pH.

Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells.

Application

Sigma 7-9® has been used:

  • as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA)
  • as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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