T1503

Trizma^® base

Primary Standard and Buffer, ≥99.9% (titration), crystalline

• Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• PubChem Substance ID: 24899973
• NACRES: NA.25

Properties

• Quality Level: 300
• description: aminopeptidase substrate
• Assay: ≥99.9% (titration)
• form: crystalline
• pH: 10.5-12
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: methanol: soluble 26 mg/mL at 25 °C; ethylene glycol: soluble 79.1 mg/mL at 25 °C; water: soluble (678 g/l at 20 °C)
• absorption: ≤0.05 at 290 nm at 40%
• application(s): diagnostic assay manufacturing
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

40% (w/w) solution in water is clear and colorless.

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma^® base has been used:

• as a component of H buffer (cell dissociation buffer)
• for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
• as an assay buffer for reconstitution of extracted and dried protein samples
• to prepare Tris-HCl buffer that is used to stabilize proteins
• as a buffer to extract carotenoid from tubers
• as a component of sample buffer during protein extraction prior to western blotting
• as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
• to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
• as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves