T1580

Anti-TDP-43 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-ALS10, Anti-TARDBP, Anti-TARDP43
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: human, mouse, rat
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 5-10 μg/mL using rat, mouse, and human kidney; indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells; western blot: 1-2 μg/mL using human U2OS cell lysates
• UniProt accession no.: Q13148
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TARDBP(23435)

Description

General description

TDP-43 (TAR DNA binding protein, TARDP) is a 414 amino acid nuclear protein and is a member of the heterogenous nuclear ribonucleoproteins (hnRNPs) family that bind single stranded RNA. It is encoded by the gene mapped to human chromosome 1p36.22. The encoded protein belongs to the family of heterogenous nuclear ribonucleoproteins (hnRNPs) that bind single stranded RNA. TDP-43 is ubiquitously expressed and is characterized with two RNA-recognition motifs and a glycine-rich C-terminal region.

Biochem/physiol Actions

Anti-TDP-43 (C-terminal) specifically recognizes human, mouse, and rat TDP-43.

Heterogeneous nuclear ribonucleoproteins (hnRNPs) play a vital role in generation and processing of RNA, including transcription, splicing, transport and stability. TDP-43 acts as a transcription regulator for human immunodeficiency virus (HIV). Abnormal phosphorylation of TDP-43 at Ser409/410 contributes to the pathology of frontotemporal lobe degeneration subtype (FTLD-U) and amyotrophic lateral sclerosis (ALS).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable