T1654

Sigma-Aldrich

Transforming Growth Factor-β1 from human platelets

TGF-β1, lyophilized powder, suitable for cell culture, 1 × 106 U/mg

Synonym(s):
TGF-β1
MDL number:
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

biological source

human

assay

≥97% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

0.04-0.3 ng/mL ED50/EC50

quality

endotoxin tested

specific activity

1 × 106 U/mg

mol wt

protein 25 kDa

packaging

pkg of 1 μg
pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μgtested (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TGFB1(7040)

General description

The biologically active form of TGF-β1 (transforming growth factor-β1 human) exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3. TGF-β1 encodes a 25 kDa peptide. TGF-β1 precursor polypeptide is characterized with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The TGF-β1 gene is mapped to human chromosome 19q13.2.

Application

Transforming Growth Factor-β1 from human platelets has been used
  • to study the induction of a-smooth muscle actin by transforming growth factor-1 (TGF-β1) in breast cancer cells.
  • to study the efficiency of TGF-β1 in promoting embryonic stem-like cell proliferation with or without feeders.
  • as control to analyse the isolated TGF-β1 HPLC using reverse-phase chromatography.
  • to induce profibrotic response in renal fibroblasts and further study functional role for Smad3 (mothers against decapentaplegic homolog 3) T388 phosphorylation.

Biochem/physiol Actions

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.
TGF-β1 plays a key role in embryogenesis and tissue homeostasis. TGF-β1 is fundamentally involved in differentiation by mediating adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, migration, death, tissue growth, and other functions in a wide range of cell types. TGF-β1 is associated with the developmental and metastasis of colorectal cancer. Downregulation of TGF-β1 might lead to many disease conditions, including tumor formation.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile, 0.1% TFA, containing 50 ug of BSA per 1 ug cytokine

Analysis Note

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

In vitro culture of embryonic disc cells from porcine blastocysts.
Hochereau-de Reviers MT and Perreau C
Reproduction, Nutrition, Development, 33.5, 475-483 (1993)
Local injection of TGF-β increases the strength of
tibial fractures in the rat
Nielsen HM, et al.
Acta Orthopaedica Scandinavica, 65 (1 , 37- 41 (1994)
Regulation of Renal Fibrosis by Smad3 Thr388 Phosphorylation
Xinli Qu, et al.
The American Journal of Pathology, 184, 944-952 (2014)
Aaron Schindeler et al.
BMC musculoskeletal disorders, 11, 105-105 (2010-06-01)
Transforming growth factor-beta (TGF-beta) and bone morphogenetic proteins (BMPs) utilize parallel and related signaling pathways, however the interaction between these pathways in bone remains unclear. TGF-beta inhibition has been previously reported to promote osteogenic differentiation in vitro, suggesting it may...
Epithelial to Mesenchymal Transition in Human Breast Cancer Can Provide a Nonmalignant Stroma
Petersen OW, et al.
The American Journal of Pathology, 162(2), 391?402-391?402 (2003)

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