T1819

Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):
Tris hydrochloride solution
NACRES:
NA.25

Quality Level

grade

BioPerformance Certified
for molecular biology

form

liquid

concentration

1 M

application(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.0

useful pH range

7.0 - 9.0

absorption

≤0.05 at 290 at 40%

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General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Kaichun Zhu et al.
Nature protocols, 1(6), 3088-3093 (2007-04-05)
This protocol describes a streamlined method of plasmid DNA extraction by continual thermal lysis, a modification of the basic boiling lysis technique, to simplify the processing of large volumes of Escherichia coli cultures. Fermented bacteria are harvested using a hollow...
Junya Yamagish et al.
Methods in molecular biology (Clifton, N.J.), 1201, 193-205 (2014-11-13)
Parasites change their transcriptional systems in different developmental stages and in response to environmental changes. To investigate the molecular mechanisms that underlie transcriptional regulation, it is essential to identify the exact positions of the transcriptional start sites (TSSs) and characterize...

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