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T1819

Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):
Tris hydrochloride solution
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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This Item
T3069T2569T2444
grade

BioPerformance Certified, for molecular biology

grade

BioPerformance Certified, for molecular biology

grade

BioPerformance Certified, for molecular biology

grade

BioPerformance Certified, for molecular biology

form

liquid

form

solution

form

solution

form

solution

concentration

1 M

concentration

2 M

concentration

1 M

concentration

1 M

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

pH

7.0

pH

8.0

pH

7.8

pH

7.6

General description

Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0. It is applicable in electrophoretic studies and capillary electrochromatography.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used:
  • as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii
  • in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies
  • for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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