This product may be stored at -20°C in aliquots at least until the recommended retest date published in the Certificate of Analysis. Avoid repeated freeze/thaw cycles.
T1952
Trichostatin A, Ready Made Solution
5 mM in DMSO, from Streptomyces sp.
Synonym(s):
TSA, [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide
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About This Item
Empirical Formula (Hill Notation):
C17H22N2O3
CAS Number:
Molecular Weight:
302.37
UNSPSC Code:
12352202
NACRES:
NA.32
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Product Name
Trichostatin A, Ready Made Solution, 5 mM in DMSO, from Streptomyces sp.
SMILES string
N(O)C(=O)\C=C\C(=C\C(C)C(=O)c1ccc(cc1)N(C)C)\C
InChI
1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+
InChI key
RTKIYFITIVXBLE-WKWSCTOISA-N
biological source
Streptomyces sp.
assay
≥98% (HPLC)
form
DMSO solution
concentration
5 mM in DMSO
technique(s)
cell culture | mammalian: suitable
antibiotic activity spectrum
fungi
neoplastics
mode of action
enzyme | inhibits
shipped in
dry ice
storage temp.
−20°C
Quality Level
1 of 4
This Item | M7449 | 647925 | SML1845 |
|---|---|---|---|
| assay ≥98% (HPLC) | assay ≥90% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) |
| biological source Streptomyces sp. | biological source - | biological source - | biological source (Thapsia garganica L seeds) |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| concentration 5 mM in DMSO | concentration 10 mM in DMSO | concentration - | concentration 10 mM in DMSO |
| form DMSO solution | form DMSO solution | form lyophilized solid | form DMSO solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Application
Trichostatin A, ready-made solution has been used:
- as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing[1]
- to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)[2]
- to treat cells for the HDAC inhibition experiments[3]
Biochem/physiol Actions
Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.
Trichostatin A is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase.
General description
Trichostatin A is a compound of primary hydroxamic acid.[4]
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Bioprobes: Biochemical Tools for Investigating Cell Function, 11, 2104-2104 (2012)
Negative regulation of human growth hormone gene expression by insulin is dependent on hypoxia-inducible factor binding in primary non-tumor pituitary cells
Vakili H, et al.
The Journal of Biological Chemistry, 287(40), 33282-33292 (2012)
Barbara Wójcikowska et al.
Frontiers in plant science, 9, 1353-1353 (2018-10-03)
Auxin is an important regulator of plant ontogenies including embryo development and the exogenous application of this phytohormone has been found to be necessary for the induction of the embryogenic response in plant explants that have been cultured in vitro.
Zn(II)-dependent histone deacetylase inhibitors: suberoylanilide hydroxamic acid and trichostatin A.
Rachel Codd et al.
The international journal of biochemistry & cell biology, 41(4), 736-739 (2008-08-30)
Suberoylanilide hydroxamic acid (SAHA, vorinostat, Zolinza) and trichostatin A (TSA) are inhibitors of the Zn(II)-dependent class I and class II histone deacetylases (HDACs), which are enzymes that operate in concert with histone acetyltransferases (HATs) to regulate the acetylation status of
M Yoshida et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 17(5), 423-430 (1995-05-01)
Reversible acetylation at the epsilon-amino group of lysines located at the conserved domain of core histones is supposed to play an important role in the regulation of chromatin structure and its transcriptional activity. One promising strategy for analyzing the precise
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