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Trizma^® hydrochloride solution

Name: Trizma<SUP>®</SUP> hydrochloride solution
Brand: SIGMA
Price: 66.04 USD

Molecular Biology, 1 M, BioReagent

Synonym(s):

Tris hydrochloride solution

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Pack Size	SKU	Availability	Price
1 L		Check Cart for Availability	$77.70 $66.04

About This Item

NACRES:

NA.25

PubChem Substance ID:

24900064

UNSPSC Code:

12161700

MDL number:

MFCD00012590

$66.04

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Properties

grade

Molecular Biology

Quality Level

200

sterility

0.2 μm filtered

product line

BioReagent

form

solution

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

pH

7.35-7.45

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

General description

Trizma^® hydrochloride solution is a useful biological buffer.

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Trizma^® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35.[1] It was used to prepare the triton extract.[2] It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis.[3] It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.[4]

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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1 of 1

This Item	T3038	T2569	T1819
Sigma-Aldrich T2663 Trizma^® hydrochloride solution	Sigma-Aldrich T3038 Trizma^® hydrochloride solution	Sigma-Aldrich T2569 Trizma^® hydrochloride solution	Sigma-Aldrich T1819 Trizma^® hydrochloride solution
grade Molecular Biology	grade Molecular Biology	grade BioPerformance Certified, Molecular Biology	grade BioPerformance Certified, Molecular Biology
concentration 1 M	concentration 1 M	concentration 1 M	concentration 1 M
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
product line BioReagent	product line BioReagent	product line -	product line -
form solution	form solution	form solution	form liquid
pH 7.35-7.45	pH 8.0	pH 7.8	pH 7.0

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites.

B E Murray et al.

Journal of clinical microbiology, 28(9), 2059-2063 (1990-09-01)

Epidemiologic evaluation of enterococci has been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations

Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis.

M Fujii et al.

Journal of bacteriology, 154(2), 831-837 (1983-05-01)

The structural gene for a thermostable protease from Bacillus stearothermophilus was cloned in plasmid pTB90. It is expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 U/mg of cell

Reconstitution and purification of the D-glucose transporter from human erythrocytes.

M Kasahara et al.

The Journal of biological chemistry, 252(20), 7384-7390 (1977-10-25)

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Global Trade Item Number

SKU	GTIN
T2663-100ML	04061838238313
T2663-10L	04061838244949
T2663-1L	04061837342721

Questions

1–2 of 2 Questions

Anonymous

9 months ago

How can I determine the shelf life / expiration / retest date of this product?

1 answer
1. Technical Support
  
  9 months ago
  
  If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
  For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
  For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
  For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
  
  Helpful?
Anonymous

a year ago

How is shipping temperature determined? And how is it related to the product storage temperature?

1 answer
1. Technical Support
  
  a year ago
  
  Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
  
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