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T2819

Sigma-Aldrich

Trizma® hydrochloride solution

pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture

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Synonym(s):
Tris hydrochloride solution
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

9.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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Trizma® hydrochloride solution pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture

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Trizma® hydrochloride solution pH 7.6, BioPerformance Certified, 1 M, suitable for cell culture

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Trizma® hydrochloride solution pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture

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Trizma® hydrochloride solution pH 7.4, BioPerformance Certified, 1 M, suitable for cell culture

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T2194

Trizma® hydrochloride solution

Premium Grade
form

solution

form

solution

form

solution

form

solution

concentration

1 M

concentration

1 M

concentration

1 M

concentration

1 M

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

pH

9.0

pH

7.6

pH

7.8

pH

7.4

General description

Tris hydrochloride is used to prepare buffer, mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used:
  • to neutralize IgG bound to protein A/G column
  • as a component in BigDye buffer for sequencing reaction
  • to block slides

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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