Trizma® hydrochloride solution

pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture

Tris hydrochloride solution

Quality Level


BioPerformance Certified
for molecular biology




1 M


cell culture | mammalian: suitable


DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)



useful pH range

7.0 - 9.0


≤0.05 at 290 at 40%

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General description

Tris hydrochloride is used to prepare buffer, mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.


Trizma® hydrochloride solution has been used:
  • to neutralize IgG bound to protein A/G column
  • as a component in BigDye buffer for sequencing reaction
  • to block slides

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Genotypic inference of HIV-1 tropism using population-based sequencing of V3
McGovern RA, et al.
Journal of Visualized Experiments, 142(46), e2531-e2531 (2010)
Using improved technology for filter paper-based blood collection to survey wild Sika deer for antibodies to hepatitis E virus
Yu C, et al.
Journal of Virological Methods, 142(1-2), 143-150 (2007)
Experimental protocol for detecting cyanobacteria in liquid and solid samples with an antibody microarray chip
Blanco Y, et al.
Journal of Visualized Experiments, 142(120), e54994-e54994 (2017)
Tris/Tris (textperiodcentered) HCl: a standard buffer for use in the physiologic pH range
Durst RA and Staples BR
Clinical Chemistry, 18(3), 206-208 (1972)
Hao Wang et al.
Plant physiology, 172(2), 1003-1018 (2016-08-18)
Post-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study using Nicotiana tabacum pectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway...

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