T3073

Tissue Preparation Solution

Name: Tissue Preparation Solution
Brand: SIGMA
Price: 398 USD

30 mL sufficient for 1,000 extractions

Synonym(s):

Direct PCR tissue preparation solution, Extract-N-Amp^™ Tissue Preparation Solution, REDExtract-N-Amp^™ Tissue Preparation Solution, SYBR^® Green Extract-N-Amp^™ Tissue Preparation Solution

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30 ML

$398.00

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About This Item

UNSPSC Code:

12352200

EC Number:

254-457-8

NACRES:

NA.55

Key Documents

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Quality Level

200

usage

30 mL sufficient for 1,000 extractions, sufficient for 1000 reactions

feature

Direct PCR

technique(s)

PCR: suitable

input

crude DNA

shipped in

wet ice

storage temp.

−20°C

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Show Differences

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This Item	XNAT	D5688	XNAT2
Sigma-Aldrich T3073 Tissue Preparation Solution	Sigma-Aldrich XNAT REDExtract-N-Amp^™ Tissue PCR Kit	Sigma-Aldrich D5688 Dilution Solution	Sigma-Aldrich XNAT2 Extract-N-Amp^™ Tissue PCR Kit
usage 30 mL sufficient for 1,000 extractions	usage sufficient for 10 reactions, 10 reactions sufficient for 10 amplifications, 10 reactions sufficient for 10 extractions, sufficient for 100 reactions, 100 reactions sufficient for 100 amplifications, 100 reactions sufficient for 100 extractions, sufficient for 1000 reactions, 1000 reactions sufficient for 1000 amplifications, 1000 reactions sufficient for 1000 extractions	usage -	usage sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions
shipped in wet ice	shipped in wet ice	shipped in -	shipped in wet ice
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

General description

The Tissue Preparation Solution is intended for use with the Extract-N-Amp^™ Tissue PCR kits, REDExtract-N-Amp^™ Tissue PCR kits, and SYBR^® Green Extract-N-Amp Tissue PCR kits. This product is intended to supplement the kit reagents, where there are many extractions.

Application

Tissue preparation solution has been used:

in combining segments of tails for genotyping[1]
to extract genomic DNA from zebrafish embryos/fin tissues
to extract DNA from ear tags, tail tips and embryonic yolk sacs of mouse embryo
for extraction of DNA from fungal spores and mycelia[2]

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

related product

Product No.

Description

Pricing

XNAT2

Extract-N-Amp^™ Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

E7526

Extraction Solution, Molecular Biology

XNAT2R

Extract-N-Amp^™ Tissue PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAT

REDExtract-N-Amp^™ Tissue PCR Kit, sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

R4775

REDExtract-N-Amp^™ PCR ReadyMix^™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

Extract-N-Amp^™ PCR ReadyMix^™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E8155

Extract-N-Amp^™ PCR Diluent, colorless, storage temp.:2-8°C

S4320

SYBR^® Green Extract-N-Amp^™ PCR ReadyMix^™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

XNATG

SYBR^® Green Extract-N-Amp^™ Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNATRG

SYBR^® Green Extract-N-Amp^™ Tissue PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

E0781

Extract-N-Amp^™ PCR ReadyMix^™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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In vitro experimental environments lacking or containing soil disparately affect competition experiments of Aspergillus flavus and co-occurring fungi in maize grains.

Titilayo D O Falade et al.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 33(7), 1241-1253 (2016-06-07)

In vitro experimental environments are used to study interactions between microorganisms, and to predict dynamics in natural ecosystems. This study highlights that experimental in vitro environments should be selected to match closely the natural environment of interest during in vitro

A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish

Varshney GK, et al.

Nature Protocols, 11(12), 2357-2357 (2016)

N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.

Soh BS

Cell Research, 24(12), 1420-1432 (2014)

Altered Developmental Expression of the Astrocyte-Secreted Factors Hevin and SPARC in the Fragile X Mouse Model.

Jessica Wallingford et al.

Frontiers in molecular neuroscience, 10, 268-268 (2017-09-14)

Astrocyte dysfunction has been indicated in many neurodevelopmental disorders, including Fragile X Syndrome (FXS). FXS is caused by a deficiency in fragile X mental retardation protein (FMRP). FMRP regulates the translation of numerous mRNAs and its loss disturbs the composition

Molecular dissection of TNFR-TNFα bidirectional signaling reveals both cooperative and antagonistic interactions with p75 neurotrophic factor receptor in axon patterning.

K D Kuhn et al.

Molecular and cellular neurosciences, 103, 103467-103467 (2020-02-01)

During neural development, complex organisms rely on progressive and regressive events whereby axons, synapses, and neurons are overproduced followed by selective elimination of a portion of these components. Tumor necrosis factor α (TNFα) together with its cognate receptor (Tumor necrosis

Protocols

REDExtract-N-Amp™ Tissue PCR Kit Protocol

Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

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Tissue Preparation Solution

30 mL sufficient for 1,000 extractions

Select a Size

T3073-30ML

$398.00

About This Item

Key Documents

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Properties

Quality Level

usage

feature

technique(s)

input

shipped in

storage temp.

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Description

General description

Application

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related product

Safety Information

pictograms

signalword

hcodes

pcodes

Hazard Classifications

target_organs

Storage Class

flash_point_f

flash_point_c

Documentation

Certificates of Analysis (COA)

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Peer Reviewed Papers

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