T3287

Sigma-Aldrich

Monoclonal Anti-Talin antibody produced in mouse

clone 8d4, ascites fluid

Synonym(s):
Monoclonal Anti-Talin
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

8d4, monoclonal

mol wt

antigen 225-235 kDa

contains

15 mM sodium azide

species reactivity

frog, chicken, human, mouse, rat

application(s)

immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:500 using cultured Rat2 cells
western blot: 1:100 using chicken gizzard extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

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General description

Monoclonal Anti-Talin (mouse IgG1 isotype) is derived from the 8d4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Talin is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin.
Talin 1 (TLN1) gene mapped to human chromosome 9p13.3, codes for a 270kDa cytoskeletal protein containing 2541 amino acids. Talin-1 is widely expressed in the kidney, liver, spleen, stomach, lung and vascular smooth muscle. The TLN1 antiparallel dimeric structure is characterized with a globular head containing four-point-one, ezrin, radixin, moesin (FERM) domain and rod domain involved in dimer formation.

Specificity

Reacts specifically with mammalian and avian talin. In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. It also localizes the slightly higher molecular weight talin in human platelets. The antibody labels focal adhesions, membrane ruffles, and ventral streaks in methanol/acetone fixed cultured fibroblasts.

Immunogen

purified chicken gizzard talin.

Application

Monoclonal Anti-Talin may be used for the localization of talin using various immunochemical assays such as immunoblot, immunocytochemistry, immunofluorescence, enzyme linked immunosorbent assay (ELISA) and for microinjection and transfection studies. The antibody recognizes native talin and can be used for immunoprecipitation.

Biochem/physiol Actions

Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques.
Talin 1 (TLN1) is a cytoskeletal protein, which plays a vital role in cancer cell adhesion, migration and invasion. It is also involved in integrin activation and it facilitates the adhesion of integrin to actin cytoskeleton. TLN1 might act as a potential prognostic biomarker in nasopharyngeal carcinoma (NPC). Overexpression of TLN1 is observed in endometrioid carcinoma, oral squamous cell carcinoma (OSCC), prostate cancer and hepatocellular carcinoma. Serum TLN1 functions as a potential biomarker of hepatocellular carcinoma (HCC). Talin 1 (TLN1) is highly expressed in glioblastoma multiforme (GBM). The Inhibition of TLN1 activity helps in diminishing malignant features of GBM cells. It also plays an important role in regulation of, Bevacizumab (anti-angiogenic agent)-resistance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Ligand-dependent localization and intracellular stability of sigma-1 receptors in CHO-K1 cells
Mavlyutov TA and Ruoho AE
Journal of Molecular Signaling, 2(1), 8-8 (2007)
Regionally-selective cell colonization of micropatterned surfaces prepared by plasma polymerization of acrylic acid and 1, 7-octadiene.
Filova E, et al.
Physiological Research, 58(5) (2009)
D R Critchley
Biochemical Society transactions, 32(Pt 5), 831-836 (2004-10-21)
The cytoskeletal proteins talin and vinculin form part of a macromolecular complex on the cytoplasmic face of integrin-mediated cellular junctions with the extracellular matrix. Recent genetic, biochemical and structural data show that talin is essential for the assembly of such...
Wonyoung Kang et al.
Oncotarget, 6(29), 27239-27251 (2015-09-05)
Glioblastoma multiforme (GBM) possesses florid angiogenesis. However, the anti-angiogenic agent, Bevacizumab, did not improve overall survival of GBM patients. For more durable anti-angiogenic treatment, we interrogated resistant mechanisms of GBM against Bevacizumab. Serial orthotopic transplantation of in vivo Bevacizumab-treated GBM...
Dipeptidyl peptidase 9 subcellular localization and a role in cell adhesion involving focal adhesion kinase and paxillin
Zhang H, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1853(2), 470-480 (2015)

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