T3320

Anti-γ-Tubulin (AK-15) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-CDCBM4, Anti-GCP-1, Anti-TUBG, Anti-TUBGCP1
• MDL number: MFCD00677366

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 48 kDa
• species reactivity: human, frog, rat
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• technique(s): immunocytochemistry: 1:1,000 using methanol-acetone fixed, cultured rat, RAT-2 cells; microarray: suitable; western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line.; western blot: 1:5,000 using Xenopus eggs extract
• UniProt accession no.: P23258
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... TUBG1(7283) , TUBG2(27175) ; rat ... Tubg1(252921) , Tubg2(680991)

Description

General description

γ-Tubulin (48 kDa) is a highly conserved protein. The microtubule cytoskeleton consists of a dynamic, highly polarized network of microtubules filaments, microtubule-associated proteins, microtubule motors, and microtubule-organizing proteins. γ-Tubulin is found as part of a large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule.

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ -Tubulin consists of two isoforms tubulinγ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ -tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Application

Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in following studies:

• Western blot.
• Immunofluorescence.

Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in:

• western blotting
• immunocytochemistry (immunofluorescence staining
• immunofluorescence

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression ofγ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable