For the dissociation of monolayer cultures, the suggested working concentration is 0.0025%. The working concentration for use with solid tissue has not been established. The concentration should be optimized by the end user.
T3568
TrypZean® bovine
recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP)
Synonym(s):
Trypsin bovine
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mg | Check Cart for Availability | $331.00 | |
| 100 mg | Check Cart for Availability | $1,640.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-650-8
MDL number:
Specific activity:
≥3,350 units/mg solid (USP)
Recombinant:
expressed in corn
$331.00
Check Cart for Availability
recombinant
expressed in corn
Quality Level
form
lyophilized powder
specific activity
≥3,350 units/mg solid (USP)
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... TRYP8(282603)
General description
TrypZean® eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
Application
Eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
TrypZean® has been used in a study to assess the use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells. [1] TrypZean® has also been used in a study to compare a recombinant trypsin with the porcine pancreatic extract on sperm used for the in vitro production of bovine embryos. [2]
Biochem/physiol Actions
For dissociation of cultured cells.
Preparation Note
Manufactured by Applied Biotechnology Institute utilizing proprietary transgenic plant protein expression system.
Other Notes
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer
Legal Information
Trypzean is a registered trademark of ProdiGene, Inc.
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information cow ... TRYP8(282603) | Gene Information - | Gene Information - | Gene Information - |
| specific activity ≥3,350 units/mg solid (USP) | specific activity ≥10,000 BAEE units/mg protein | specific activity - | specific activity 1,000-2,000 BAEE units/mg solid |
| recombinant expressed in corn | recombinant - | recombinant - | recombinant - |
| form lyophilized powder | form essentially salt-free, lyophilized powder | form liquid | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. - |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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240 recombinant bovine trypsin made in maize inactivates bovine herpes virus-1 adsorbed to the bovine Zona Pellucida
Seidel, G., et al.
Reproduction, Fertility, and Development, 19, 236-236 (2006)
Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
Pedro P Carvalho et al.
Cytotherapy, 13(5), 594-597 (2011-01-05)
Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells
K J Mattson et al.
Theriogenology, 69(6), 724-727 (2008-02-19)
The objective was to compare the effects of treating bovine semen with two trypsin products (the porcine pancreas extract and a recombinant) and a control (no trypsin) on in vitro embryo production. Our hypothesis was that the trypsin treatments would
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T3568-100MG | 04061835559299 |
| T3568-10MG | 04061832707938 |
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What should be the working concentration (mg/ml)? thank you!
1 answer-
Helpful?
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