T3702

Sigma-Aldrich

Anti-TMP21 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Transmembrane trafficking protein, Anti-TMED10, transmembrane emp24-like trafficking protein 10, Anti-Transmembrane protein Tmp21 21 kDa, Anti-p24delta
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~21 kDa

species reactivity

human, rat (predicted), mouse (predicted)

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

application(s)

western blot: 1-2 μg/mL using HepG2 cell lysate

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TMED10(10972)
mouse ... Tmed10(68581)
rat ... Tmed10(84599)

General description

Transmembrane protein, 21KD (TMP21) gene is mapped on the human chromosome at 14q24.3. TMP21 belongs to the p24 family and is a type I transmembrane protein. It is located in the endoplasmic reticulum (ER) and the Golgi apparatus.

Specificity

Anti-TMP21 (N-terminal) specifically recognizes human TMP21.

Application

Anti-TMP21 (N-terminal) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Transmembrane protein, 21KD (TMP21) has been recently linked to the metabolism of Alzheimer′s disease amyloid β (Ab) peptide. It is a member of the heteromeric secretase complex that distinctively regulates γ-secretase cleavage without affecting its ε-secretase cleavage. TMP21 is destabilized from the complex in the absence of the presenilins PS1, PS2, and pen-2. It might modulate intramembrane proteolysis controlling g-secretase activity and thus preventing the over-production of Ab peptide. Overexpression of TMP21 gene does not alter γ -secretase activity, but suppression of TMP21 gene increases Aβ40 and Aβ42 peptides. TMP21 appears to be independent of its role in protein transport from the endoplasmic reticulum (ER) to the Golgi and are involved in vesicular trafficking.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
TMP21 transmembrane domain regulates gamma-secretase cleavage
Pardossi P R, et al.
Test, 284(42), 28634-28641 (2009)
Expression of tmp21 in normal adult human tissues
Xie J, et al.
International Journal of Clinical and Experimental Medicine, 7(9), 2976-2976 (2014)
TMP21 regulates A beta production but does not affect caspase-3, p53, and neprilysin
Dolcini V, et al.
Biochemical and biophysical research communications, 371(1), 69-74 (2008)
TMP21 is a presenilin complex component that modulates gamma-secretase but not ?-secretase activity
Chen F, et al.
Nature, 440(7088), 1208-1212 (2006)

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