Tyrosinase is soluble (2 mg/mL) in 50 mM potassium phosphate buffer, pH 6.5, yielding a clear brown solution.
The use of other buffers, such as phosphate buffer has not been evaluated. For optimal results and to minimize the risk of issues, we recommend closely following the provided instructions.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/407/287/t3824dat-mk.pdf
T3824
Tyrosinase from mushroom
lyophilized powder, ≥1000 unit/mg solid
Tyrosinase from mushroom
Synonym(s):
Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25000 units | Check Cart for Availability | $163.00 | |
| 50000 units | Check Cart for Availability | $278.00 | |
| 250000 units | Check Cart for Availability | $1,150.00 | |
| 500000 units | Check Cart for Availability | $1,730.00 | |
| 1000000 units | Check Cart for Availability | $2,710.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥1000 unit/mg solid
Biological source:
fungus (mushroom)
biological source
fungus (mushroom)
Quality Segment
form
lyophilized powder
specific activity
≥1000 unit/mg solid
mol wt
119.5 kDa by electrophoresis, 128 kDa by sedimentation velocity diffusion, 133 kDa by LS
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase with activity for catechols and cresol. Tyrosinase participates in the conversion of tyrosine to dihydroxyphenylalanine (DOPA), and polymerization of DOPA leads to melanin formation. Tyrosinase has a role in browning reactions. The enzyme is said to have two binding sites for aromatic substrates and a distinct binding site for oxygen-copper.
Application
Biochem/physiol Actions
Converts tyrosine to L-DOPA or tyramine to dopamine
Other Notes
One unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine.
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This Item | |||
|---|---|---|---|
| specific activity ≥1000 unit/mg solid | specific activity ≥20 units/mg protein | specific activity 100,000-250,000 units/g solid (without added oxygen) | specific activity ≥30 units/mg solid |
| biological source fungus (mushroom) | biological source fungus (Trichoderma viride) | biological source - | biological source fungus (Dactylium dendroides) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) - |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| mol wt 119.5 kDa by electrophoresis, 133 kDa by LS, 128 kDa by sedimentation velocity diffusion | mol wt 112 kDa | mol wt 160 kDa | mol wt - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T3824-25KU | 04061835559305 |
| T3824-500KU | 04061832731575 |
| T3824-50KU | 04061835546411 |
| T3824-1MU | 04061838174321 |
| T3824-250KU | 04061835546404 |
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Is it possible to use an equivalent buffer, such as phosphate buffer (Na/K), instead of the recommended potassium phosphate buffer to reconstitute the enzyme?
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Is it possible to reconstitute T3824-25KU with a solution other than potassium phosphate buffer?
1 answer-
The enzymatic assays are performed using potassium phosphate buffer as a solvent. Unfortunately, no other solvent was tested in-house. An online search indicates that water may be used to initially reconstitute the enzyme and then further dilute to the solution suitable for the application.
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Does the solid contain any copper specie?
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Yes, all native forms of Tyrosinase from mushroom contain Copper. Please see the link below to review additional information regarding the structure and sequence information of Tyrosinase from mushroom.
https://www.rcsb.org/structure/2Y9XHelpful?
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Is this Tyrosinase from mushroom food grade?
1 answer-
This product is not food grade. Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals. Currently, a food grade product option is not available.
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When measuring the activity of Tyrosinase, should we use the highest change observed every minute between Tyrosinase and the mixture (with L-tyrosine) as the activity, or the average change over the measured time?
1 answer-
Refer to the protocol of the enzymatic assay of Tyrosinase as described in the reference: Duckworth, H. W. and Coleman, J. E. (1970) Journal of Biological Chemistry, 245, 1613-1625. The protocol can be accessed at https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/776/246/tyrosinase.pdf
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Is this tyrosinase is derived from Agaricus bisporus or another mushroom species?
1 answer-
Yes, this product is derived from Agaricus bisporus.
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Can you please tell me 25000 units tyrosinase in milligrams?
1 answer-
This product is sold by activity. The minimum activity specification for this material is 1000 units per milligram. The weight of this product can be determined using the activity value reported on the lot specific Certificate of Analysis(COA). For example, the sample COA has a activity value of 6540 units/mg. The amount of powder in the 25,000 unit package will be 3.82mg.
Please see the link below or navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a sample or lot specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/t3824#product-documentationHelpful?
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Hi, How much is the total protein amount of T3824 (25KU) per vial?
1 answer-
The protein content of this product is not determined. This product is tested and released based on the activity per milligram solid. Should the protein content be required, please see the link below to review several kits that may be of interest:
https://www.sigmaaldrich.com/US/en/product/compare?term=total%20protein%20kit&facet%5B0%5D=facet_product_category%3Akits&focus=products&page=1&perpage=30&sort=relevance&type=product&products=TP0300%3ASIGMA%3Asial%2CTP0200%3ASIGMA%3Asial%2CTP0100%3ASIGMA%3AsialHelpful?
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What solution is best to be used to reconstitute the T3824-25KU? and what is the volume and concetration of that soluation?
1 answer-
Tyrosinase is soluble at 2 mg/mL in 50 mM potassium phosphate buffer. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/377/683/t3824dat-ms.pdfHelpful?
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I see that the approximate activity of Product T3824, Tyrosinase from mushroom, is ?1000 unit/mg solid, but how can I find the lot specific activity of this tyrosinase?
1 answer-
The certificate of analysis for the lot will report the exact tyrosinase activity for a particular batch. The lot number can be found on the label of the bottle, or you can call technical service (800-325-5832) for a sample lot number to view the online certificate of analysis.
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